FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043027
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG29100.14243570936654287No Hit
ATCTATTATAGTGGGATTACCTACTACAACCCGTCCCTCAAGAGTCGAGT27650.13533839738779763No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGT27410.13416366988786738No Hit
GTATAGCAGTGGCTGGTACCAAACCATACTACTACTACGGTATGGACGTC25730.12594057738835562No Hit
ATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA25010.12241639488856486No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACAC23710.116053287597276No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22270.1090049225976945No Hit
CTATTATAGTGGGATTACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA21260.10406127770215469No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC36500.048.1274034
ATCAACG37350.047.2248883
CAACGCA38350.045.805745
AACGCAG40600.043.089926
TATCAAC42750.041.289952
ACGCAGA43250.040.2832687
CGCAGAG47500.036.982118
GCAGAGT56050.031.597669
GTATCAA62600.031.357651
GTACATG45600.029.2789751
TACATGG46750.027.4317052
CATGGGG35800.022.121024
TAATACG1650.00270977321.816324
CAGAGTA54600.021.2289589
TACACCG2404.6079127E-520.9982095
GTATTAA11300.020.4371591
TATTACG2257.167188E-419.2124672
ACATGGG69600.018.6191023
TAATACT11500.018.1549724
CTAGTAC3653.1946838E-617.7519113
Produced by FastQC (version 0.11.5)