FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2043027
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG24910.12192692509692725No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGAGT24460.11972431103455802No Hit
ATCTATTATAGTGGGATTACCTACTACAACCCGTCCCTCAAGAGTCGAGT23630.11566171176396593No Hit
GTATAGCAGTGGCTGGTACCAAACCATACTACTACTACGGTATGGACGTC22290.10910281655602203No Hit
ATAATAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA20490.10029236030654515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22650.039.4097944
ATCAACG23800.037.5055433
CAACGCA25100.035.2762225
AACGCAG27400.031.7896336
TATCAAC29650.031.3195652
ACGCAGA27500.030.888737
GTACATG45850.030.0157221
TACATGG47200.028.2150252
CGCAGAG29750.028.0686658
GTATTGG9100.026.921071
GCAGAGT38600.021.8197239
CATGGGG34450.021.1048774
TATACTG11500.020.031015
ACATGGG65600.019.423173
GTATTAG7100.019.281911
GTATCAA63100.018.3844781
CCTAGGT12150.017.774432
TATTGAG13400.017.7280065
CAGAGTA37100.017.6570539
TACACGG28150.017.3892635
Produced by FastQC (version 0.11.5)