FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916999
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATACACTGGTACCAGCAGAAACCAGGTCAGTCTCCAAAGCTCCTCAT31590.3444932873427343No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT25470.27775384705981143No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17670.19269377611098812No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13740.14983658651754253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12030.13118880173260822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10940.11930220207437521No Hit
GTCTCTGGTGGCTCCATCAGTGATCACTACTGGAGCTGGATCCGGCAGCC10490.11439489028886617No Hit
GTATTACTGTCATCAGCGTGGTAGTTTACCGATCACCTTCGGCCAAGGGA10430.11374058205079832No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10360.11297722243971911No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10140.11057809223347027No Hit
CACTGGTACCAGCAGAAACCAGGTCAGTCTCCAAAGCTCCTCATCAAGTA9800.10687034555108565No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9750.1063250886860291No Hit
TCTCAGGGGTCCCCTCGAGGTTCAGTGGCAGTGGATCTGGGACCGATTTC9280.10119967415449743No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)