FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922769
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15260.1653718319536092No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14450.1565939037830703No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14150.1533428192754633No Hit
ATAATAGACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATGTAGG13490.14619043335872792No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13480.146082063875141No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA13180.14283097936753403No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13000.14088032866296982No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12900.13979663382710084No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12050.13058522772221434No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGACGACACGGCCGTCTATTA11900.12895968546841083No Hit
ACATGGAACTGAGCAGCCTGAGATCTGACGACACGGCCGTCTATTATTGT11390.12343284180547894No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG10970.11888132349482915No Hit
GTCTATTATTGTGCGGGCGATCCCGGATATTGTCGTGGCAATAATTGTTA9850.10674394133309638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG15250.065.667133
TCAACGC15600.065.0755844
CAACGCA15800.064.251855
AACGCAG17600.057.6775056
TATCAAC17550.057.5093152
GGTATCA7500.051.5717241
CGCAGAG21450.047.3251348
ACGCAGA22900.044.3285647
GCAGAGT23900.042.45549
GTATCAA26300.040.0350841
TATACCG750.001589016438.128795
GTACATG19900.033.1141741
TACACCG2101.6500053E-830.639215
ACATGGG24700.025.7740633
GTACACG7600.025.3982935
CAGAGTA23100.024.7468839
TACACGG7900.024.4324766
TACATGG27100.023.5080662
GTATATA5600.023.0230921
TAGGTTG3203.9262886E-822.3410915
Produced by FastQC (version 0.11.5)