FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873986
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30260.3462298023080461No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCACGAGGTTGTTG21260.243253324423961No Hit
GTCAGTGACCATCTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATA20300.23226916678299195No Hit
TCTCTGGGCTCCGGGCTGAAGATGAGGCGCAGTATTTTTGCAGCTCATAT19630.2246031400960656No Hit
CTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATAACTTTGTCTCCT19410.22208593730334353No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC19310.22094175421574258No Hit
CTCCTGGACAGTCAGTGACCATCTCCTGCACTGGATCCAGCAGTGACGTT17830.2040078445192486No Hit
GTCTCTGGTGGCTCCATCAGTGGAAACTACTGGACCTGGATCCGGCAGCC17700.20252040650536735No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17370.1987446023162842No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCAC16200.1853576601913532No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15860.18146743769350998No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15430.1765474504168259No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15360.17574652225550524No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG15320.17528884902046485No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15100.17277164622774277No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14770.16899584203865964No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14740.16865258711237938No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGTTTCAGGGAGA14390.16464794630577606No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13620.15583773653124877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12790.14634101690416093No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12390.14176428455375717No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12360.14142102962747688No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12230.13993359161359564No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12160.13913266345227496No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11980.13707313389459327No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11800.1350136043369116No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11780.13478476771939138No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11680.13364058463179043No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11550.13215314661790922No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11520.1318098916916289No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCACGAGGTTGTTGC11320.12952152551642704No Hit
GCAAAAATACTGCGCCTCATCTTCAGCCCGGAGCCCAGAGACGGTCAGGG11280.12906385228138667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11180.1279196691937857No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11170.12780525088502562No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11160.12769083257626554No Hit
GTATTTTTGCAGCTCATATGCAGGCAGCAACAACCTCGTGGTGTTCGGCG10980.12563130301858383No Hit
GTGTATTACTGTGCTGAAGTTCATCCGGGGTATTACGATGTTTTGACTGG10920.12494479316602326No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10830.12391502838718241No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10820.1238006100784223No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10780.12334293684338193No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10690.12231317206454109No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10180.11647783831777625No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9930.11361738059877388No Hit
GGATCCAGCAGTGACGTTGGTAGTTATAACTTTGTCTCCTGGTACCAACA9730.111329014423572No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT9540.1091550665571302No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9440.10801088346952926No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9360.10709553699944852No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG8830.1010313666351635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.075.774771
GTATCAA19050.068.420911
GAATACG2600.065.959949
ATCAACG20650.062.3794173
TCAACGC20800.061.876394
CAACGCA21350.060.282385
TATCAAC22050.058.4322172
AACGCAG22200.057.9616
GTGCTAG3500.053.2007521
CGCAGAG27000.047.921588
ACGCAGA28450.045.2279177
GCAGAGT30050.042.8026289
GCTAGAG4550.039.320363
CTAGAGA5250.038.1341864
AGAATAC5000.035.7426158
GTACACG6750.033.8970575
TGCTAGA6350.031.5625952
TATGTAC7850.029.1788862
ACACGGG7850.029.1404767
TATTGAG11800.029.0853985
Produced by FastQC (version 0.11.5)