FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873986
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28560.32677868981883007No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCACGAGGTTGTTG21630.24748680184808453No Hit
TCTCTGGGCTCCGGGCTGAAGATGAGGCGCAGTATTTTTGCAGCTCATAT18130.20744039378205142No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC17880.20457993606304908No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCAC17410.1992022755513246No Hit
GTCAGTGACCATCTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATA17200.1967994910673626No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17040.1949687981272011No Hit
GTCTCTGGTGGCTCCATCAGTGGAAACTACTGGACCTGGATCCGGCAGCC16990.19439670658340064No Hit
CTCCTGGACAGTCAGTGACCATCTCCTGCACTGGATCCAGCAGTGACGTT16830.19256601364323914No Hit
CTCCTGCACTGGATCCAGCAGTGACGTTGGTAGTTATAACTTTGTCTCCT16670.19073532070307764No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15390.17608977718178553No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14880.1702544434350207No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13870.15869819425025114No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT13800.15789726608893048No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13610.1557233182224887No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGTTTCAGGGAGA13580.1553800632962084No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13370.15297727881224643No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG13030.14908705631440322No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCACGAGGTTGTTGC11880.13592895080699233No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11780.13478476771939138No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11570.1323819832354294No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11560.1322675649266693No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11460.13112338183906835No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11360.12997919875146743No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11270.12894943397262656No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11030.1262033945623843No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11030.1262033945623843No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10980.12563130301858383No Hit
GTATTTTTGCAGCTCATATGCAGGCAGCAACAACCTCGTGGTGTTCGGCG10960.12540246640106362No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10920.12494479316602326No Hit
GTGTATTACTGTGCTGAAGTTCATCCGGGGTATTACGATGTTTTGACTGG10680.12219875375578099No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10520.12036806081561946No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10330.11819411294917768No Hit
GCAAAAATACTGCGCCTCATCTTCAGCCCGGAGCCCAGAGACGGTCAGGG10030.11476156368637484No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9610.10995599471845087No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT9580.1096127397921706No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9540.1091550665571302No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9530.10904064824837012No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9360.10709553699944852No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9160.10480717082424662No Hit
GGATCCAGCAGTGACGTTGGTAGTTATAACTTTGTCTCCTGGTACCAACA8900.10183229479648415No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8860.10137462156144378No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8840.10114578494392358No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8830.1010313666351635No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8820.1009169483264034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG11750.068.643563
TCAACGC11850.068.064294
CAACGCA12900.062.5241785
GTATCAA13500.061.879081
GAATACG2650.059.7650649
TATCAAC13950.058.8505172
AACGCAG13900.058.0061156
CGCAGAG15900.051.6152768
GGTATCA5200.048.4712641
GTGCTAG4050.044.4533651
ACGCAGA18300.044.452677
GCAGAGT19800.041.4486359
AACGCCG800.002104758536.0072255
GCTAGAG5500.032.733843
AGAATAC5250.030.1671288
GTACTAG1703.1729942E-629.6530111
TGCTAGA6250.028.8057792
TCTACAC7300.028.608483
CTAGAGA5550.028.5462684
ACATGGG22900.027.988153
Produced by FastQC (version 0.11.5)