FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369304
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA9790.2650932565041267No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG9260.2507419361826571No Hit
GTATAGTATGTGCTACTATCACCGCTATAAATAAGTGAGACCCACTCCAG8880.24045231029179212No Hit
ATACTATACAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATT8870.24018153066308517No Hit
GTAATATACGCCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATACA8710.2358490566037736No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8700.23557827697506661Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.18792106232263936No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6250.1692372679418582Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT5980.16192621796676993Clontech Universal Primer Mix Long (96% over 26bp)
CTATACAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCCA5820.15759374390745834No Hit
GTATATTACTGTGCGGCCAACCGCCTGCCATGGGCTTTTGATATCTGGGG4510.12212161254684488No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT4500.1218508329181379No Hit
GTCTGTATAGTATGTGCTACTATCACCGCTATAAATAAGTGAGACCCACT4090.11074886814115201No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT4090.11074886814115201No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT4060.1099365292550311No Hit
ATATACGCCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATACAGCG4020.10885341074020319No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3830.10370859779477071No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA3830.10370859779477071No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG3790.10262547927994282No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3750.10154236076511491Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAG3710.10045924225028703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)