FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369304
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA10520.2848601693997357No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9040.2447847843511037No Hit
ATACTATACAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATT8380.2269133288564435No Hit
GTATAGTATGTGCTACTATCACCGCTATAAATAAGTGAGACCCACTCCAG8340.22583021034161557No Hit
GTAATATACGCCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATACA8160.22095617702489007No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG8130.22014383813876914No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6590.1784437753178953No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6260.16950804757056517No Hit
CTATACAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCCA5960.16138465870935598No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5540.1500119143036631No Hit
GTATATTACTGTGCGGCCAACCGCCTGCCATGGGCTTTTGATATCTGGGG4940.13376513658124473No Hit
GTCTGTATAGTATGTGCTACTATCACCGCTATAAATAAGTGAGACCCACT4630.12537096809132856No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA4090.11074886814115201No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3940.10668717371054741No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT3810.10316703853735676No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA3760.10181314039382189No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA3750.10154236076511491No Hit
GTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAG3740.10127158113640794No Hit
ATATACGCCCGTGTCCTCGGCTCTCAGGCTGTTTATTTGCAGATACAGCG3730.10100080150770097No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG3700.10018846262158006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG1201.0021195E-535.9948549
TCTACAC3150.034.2947463
CTTCGCA850.002836286833.877519
TACCCTA1601.9748331E-631.504035
TATGCTA2105.2887117E-727.4246529
TATACGC5000.027.3634995
ATACGCC5100.026.8196966
GGCAGCG1658.818047E-526.1780787
GTATAGT6900.026.0973631
TATACCC1400.001037798625.7210583
GTAATAT6750.025.6102121
TACGCCC5350.025.5664397
AATATAC6500.025.4836353
TAGTATG6700.024.719584
CGCCCGT5300.024.4493379
TAATATA7700.024.3213862
GGGTACG1801.5863978E-424.0095751
TGTGCTA7550.023.8376529
CTTAGAA2151.9579415E-523.4512142
CTAGCAC2151.9599955E-523.4480363
Produced by FastQC (version 0.11.5)