FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214887
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC46810.38530332450672367No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCT42170.347110472002746No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCGAGAGTGTTG36000.29632385563431No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34810.2865287059619537No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31950.26298742187545016No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG29380.24183319107044526No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC28930.23812914287501635No Hit
CTCCTGGACAGTCGATCACCCTCTCCTGCACTGGAACCAGCAGTGACATT28690.23615365050412093No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT27880.229486363752349No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA27040.2225721404542151No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26770.22034971153695776No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26040.21434092224215093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.20989606440763625No Hit
GATTATTACTGCAGCTCATATACAACCACCAACACTCTCGAGGTGTTCGG25500.20989606440763625No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT24710.20339340202010558No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23330.192034320887457No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22950.18890645796687264No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA22810.18775408741718366No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT22240.18306229303630708No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA22040.18141604939389425No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA21750.17902899611239562No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21520.17713581592362088No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC21020.1730202068175888No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG20910.17211477281426174No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20790.17112702662881404No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTC20670.17013928044336635No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT20350.16750529061550581No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20120.16561211042673105No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG19740.16248424750614665No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19580.1611672525922164No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19070.15696933130406365No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG18540.15260678565166966No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA18450.1518659760125839No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA18340.15096054200925682No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT18170.14956123491320591No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT17800.14651568417474217No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT17120.14091845579053855No Hit
GTCAGTAATCGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTC17090.14067151924417662No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16430.13523891522421427No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16400.13499197867785234No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA15550.1279954431975978No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG15500.12758388228699458No Hit
CCTCGAGAGTGTTGGTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCG15230.12536145336973725No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG15150.12470295591277213No Hit
GCTCATATACAACCACCAACACTCTCGAGGTGTTCGGCGGAGGGACCAAG15150.12470295591277213No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC15070.124044458455807No Hit
CTCTCGAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC14340.11803566916100015No Hit
ATTACTGACAGCATAAATCATGAGTTTGGGGGCTTTGCCTGGTTGTTGTT14250.11729485952191437No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14240.11721254733979374No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13800.1135908113264855No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13430.11054526058802176No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13260.10914595349197084No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA12730.10478340783957685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.10437184692897364No Hit
GTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCC12670.10428953474685299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.10280791546868144No Hit
TATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG12470.10264329110444016No Hit
CCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGC12430.10231404237595759No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG12370.10182016928323376No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12310.1013262961905099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.076.897081
GTATCAA36700.063.5511171
ATCAACG38900.058.6688423
TCAACGC39950.057.1268584
TATCAAC40150.056.7166632
CAACGCA42200.054.0809975
AACGCAG43600.052.3444446
ACGCAGA48050.047.34697
CGCAGAG48750.046.667058
GCAGAGT56700.040.250769
GAATACG6350.034.0130359
TACATGG39150.032.0277672
GTGCTAG6550.031.9700581
GTACATG39000.031.6605321
TATTGAG20700.030.9540565
CAGAGTA54200.029.2227639
TACGACG1255.3700304E-428.7977035
TTATTGA23300.028.7359074
ATTGAGT15050.027.7452956
GTGTAGA6000.027.6798231
Produced by FastQC (version 0.11.5)