FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214887
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC40720.3351752055952529No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAATTATGTCTCCT35330.290808939432227No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTCGAGAGTGTTG29960.24660729763344244No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28110.23137954394112376No Hit
CTCCTGGACAGTCGATCACCCTCTCCTGCACTGGAACCAGCAGTGACATT26240.21598716588456376No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC26050.21442323442427158No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25650.21113074713944588No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG24300.20001860255315926No Hit
GATTATTACTGCAGCTCATATACAACCACCAACACTCTCGAGGTGTTCGG23590.1941744376225937No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23280.19162275997685382No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22190.18265073212570387No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22010.18116911284753234No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA21770.17919362047663692No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20760.1708800900824521No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20380.16775222716186772No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA19540.16083800386373384No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19140.15754551657890817No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT19050.15680470693982237No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA18570.1528537221980316No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18170.14956123491320591No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18020.14832655218139632No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA17890.14725649381382794No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17860.14700955726746603No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA17800.14651568417474217No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTC17650.14528100144293254No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT17390.14314088470779587No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG16910.13918989996600506No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT16880.13894296341964316No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16750.1378729050520748No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC16180.13318111067119823No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG15410.1268430726479088No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15400.12676076046578819No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15100.12429139500216893No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT14850.1222335904491529No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14620.12034041026037813No Hit
GTCAGTAATCGGCCCTCAGGGGTTTCTCATCGCTTCTCTGGCTCCAAGTC14460.11902341534644786No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14100.11606017679010477No Hit
GCTCATATACAACCACCAACACTCTCGAGGTGTTCGGCGGAGGGACCAAG13790.11350849914436487No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT13600.11194456768407268No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12940.10651196366411034No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12760.10503034438593876No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12600.1037133494720085No Hit
CCTCGAGAGTGTTGGTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCG12270.10099704746202734No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG12180.10025623782294155No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG20450.059.136433
TCAACGC20550.057.7977944
GTATCAA22600.056.4423071
TATCAAC22900.054.381322
GGTATCA7900.049.261381
CAACGCA25450.046.9525765
AACGCAG26250.045.521646
ACGCAGA26900.043.886487
GAATACG6200.042.9585089
CGCAGAG27150.042.6869548
GTGCTAG7500.037.475141
GTACATG38300.035.3752061
TACATGG38650.034.641972
CTAAACG850.002839834733.875073
GCAGAGT35150.033.381169
TATTGAG21350.032.0305825
TCTACAC16600.030.788563
ACATGGG43550.030.2483773
AATCCGC3151.8189894E-1229.70796
TTATTGA23250.029.413034
Produced by FastQC (version 0.11.5)