FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1900102
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATGGCGGTGTCCGAGGCCCTCAGGGTGCTCCAGTGAAGGTAGG41540.21861984251371766No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT40870.21509371602156094No Hit
GTATTACTGTCAGCACTATCGTACCTCATTCACTTTCGGCCCTGGGACCA39080.20567316912460487No Hit
ATCGTACCTCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA36930.19435798709753477No Hit
ATCTATTACTGTGCGACACTAGATTACTACTACTATTACGGTATGGACGT34790.18309543382407892No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT26760.14083454467181236No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT23570.12404597226885715No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22320.11746737806707219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22190.11678320427008655No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22020.1158885154586438No Hit
GTGTTAGTAGCGTCTACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCT20670.10878363372071605No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC20600.1084152324454161No Hit
GTCTGGAGCAGAGGTGAAAAAGCCCGGGGAGTCTCTGAAGATCTCTTGTA20340.10704688485144481No Hit
AGGTACGATAGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGT20130.10594168102554494No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)