FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1604415
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG101760.6342498667738709No Hit
GATTATTACTGTGCGGCATGGGATGACAGCCTCAGTGGTCATTATGTCTT87000.5422537186451136No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC68250.425388693075046No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT65160.40612933686109887No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT57590.3589470305376103No Hit
CCTCATTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC56390.35146766890112596No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA55740.34741634801469695No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA48360.30141827395031834No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT46980.29281700806836136No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46410.2892643112910313No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC39890.2486264463994665No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC38690.24114708476298216No Hit
TTATTACTGTGCGGCATGGGATGACAGCCTCAGTGGTCATTATGTCTTCG38630.24077311668115794No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC38360.23909026031294894No Hit
ACCCAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATAATGACCACTGA37830.23578687559016837No Hit
CTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT37800.23559989154925626No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATA37690.23491428339924522No Hit
GTCCAGGAACCAGGTCTCCCTGTACCTGACCTCTGTGACCGCCGCGGACA37670.23478962737197048No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG36410.22693629765366194No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35510.22132677642629867No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC34200.2131618066398033No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG32610.20325165247146157No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA31820.19832773939410936No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT31600.19695652309408726No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC31580.19683186706681255No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30860.19234425008492192No Hit
CAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA30510.190162769607614No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA30270.18866689728031713No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29110.18143684769838228No Hit
ACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT28900.18012795941199752No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG27970.17433145414372217No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC27580.17190066161186476No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT27170.16934521305273262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26870.16747537264361154No Hit
TCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC26440.16479526805720465No Hit
CATCTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG25710.1602453230616767No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGGTACAGGGAGA25220.1571912503934456No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT24400.1520803532751813No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG24280.15133241711153286No Hit
TCCTCACCCTCCTCATTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG24200.1508337930024339No Hit
GACCCAGGACGGTGACCTTGGTCCCGGTTCCGAAGACATAATGACCACTG24190.15077146498879654No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT23720.14784204834784018No Hit
GTCTATTACTGTACGAGGGAGTTATATGACAATAGTGGTTATCTCCCCCG22770.14192088705229008No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCGGCATGGGATGACAGCCTCA22640.14111062287500428No Hit
GATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG22530.14042501472499322No Hit
GAGGTAATACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA22490.14017570267044374No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22450.13992639061589426No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21800.13587506972946525No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21400.13338194918397048No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT21160.1318860768566736No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20810.12970459637936568No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG20800.1296422683657283No Hit
GTATTACCTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCT20460.12752311590205775No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATGATCAGCGGCCCT20410.1272114758338709No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC20160.12565327549293667No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG20110.12534163542474983No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG19960.1244067152201893No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC19590.12210057871560663No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG19540.12178893864741977No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19240.1199190982382987No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTC19170.1194828021428371No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19060.11879719399282605No Hit
CTACTATGGTGTCGACGTCTGGGGCCAAGGGACCACGGTCACCGTGTCCT18860.11755063372007866No Hit
TTCCTGGACTGGTCAATCGATATGCTGACTCGACTCCTGAGGGACGGATT18320.11418492098366072No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG18210.11349931283364965No Hit
CCATAGTAGTAGCGGGGGAGATAACCACTATTGTCATATAACTCCCTCGT17900.11156714441089119No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17600.10969730400177012No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTT17590.10963497598813275No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG17210.10726651146991271No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC17200.10720418345627535No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCATTCACTG17040.10620693523807742No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16800.10471106291078057No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16720.10421243880168161No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16610.10352683065167055No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG16580.10333984661075844No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16580.10333984661075844No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG16410.10228027037892315No Hit
GCATGGGATGACAGCCTCAGTGGTCATTATGTCTTCGGAACCGGGACCAA16310.10165699024254947No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA16200.1009713820925384No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)