FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559353
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC49150.3151948275983693No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCTTG38960.24984721227329537No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA33300.21355010699950555No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG30540.19585045849143845No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30200.1936700670085606No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28530.18296049707795478No Hit
ATAATAGACAGCCGTGTCCTCGACTCTCAGAGAGTTCATTTGCAGATACA28050.17988229733742137No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT25520.16365761953835983No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25290.16218264882935424No Hit
GATTATTACTGCAGCTCATCTACAAACAAGAACACTTGGGTGTTCGGCGG24080.15442302031675958No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23970.15371759954288736No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTT23620.1514730788987484No Hit
CTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATAACTATGTCTCCT23500.15070352896361505No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCTTGT23380.14993397902848168No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22880.14672752096542604No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGGCCCCAA22840.1464710043203816No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22730.14576558354650937No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22660.14531667941768156No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCC22230.1425591254834537No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21790.13973744238796476No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA20950.13435059284203127No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT20880.1339016887132035No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19380.12428231452403657No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19340.12402579787899211No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19190.12306386046007543No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19190.12306386046007543No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT18730.12011391904206423No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18560.11902372330062531No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18470.11844656084927531No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18330.11754875259161973No Hit
GTCTATTATTGTGCAAGAGATTTAAGGGGATCCAACCACTGGGGCCAGGG18210.11677920265648638No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTG18140.1163302985276586No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18110.11613791104387525No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT17900.11479119865739189No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17280.11081519065920288No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC17230.11049454485289732No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17050.1093402199501973No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16840.10799350756371393No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG16780.10760873259614726No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16670.106903311822275No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG16530.10600550356461942No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16490.10574898691957499No Hit
GGTATCAACAACACCCAGGCAAAGGCCCCAAACTCATGATTTTTGATGTC16480.10568485775831386No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16360.10491530782318051No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATA15830.10151646227634153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC32500.056.9279064
ATCAACG32700.056.5797273
CAACGCA34400.053.992915
AACGCAG35950.051.6649786
TAGATCG450.00991245847.9936945
ACGCAGA40000.046.2539257
CGCAGAG40200.046.202888
GAATACG6150.040.970239
GTGCTAG7500.038.4924621
CAGCCGT13100.036.8195889
GCAGAGT50650.036.528279
TAGACGA800.002108697535.995275
ACAGCCG14250.034.3533828
AATAGAC14400.033.9955333
ATAGACA15800.033.7170874
TAGACAG16150.033.432145
ATAGGTG11950.031.326434
ATAATAG15950.030.7698351
TAATAGA16300.030.500932
CAGAGCG12250.029.9715719
Produced by FastQC (version 0.11.5)