FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559353
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC42180.2704968021993737No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCTTG32670.20950996984005543No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA26830.1720585396635656No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG25620.16429891115097095No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25260.16199026134557087No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25190.16154135721674312No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24040.15416650367171514No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA23500.15070352896361505No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT22610.144996033611376No Hit
ATAATAGACAGCCGTGTCCTCGACTCTCAGAGAGTTCATTTGCAGATACA22140.1419819630321037No Hit
GATTATTACTGCAGCTCATCTACAAACAAGAACACTTGGGTGTTCGGCGG21970.1408917672906648No Hit
CTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATAACTATGTCTCCT21510.1379418258726536No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20760.13313213877807012No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGGCCCCAA20710.1328114929717646No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCCC20120.12902787245735892No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTT19810.12703986845826443No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19550.1253725102654755No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18730.12011391904206423No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCTTGT18540.11889546497810309No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18370.11780526923666418No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT17590.1128031946582974No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16900.10837828253128061No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16810.10780112007993058No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16690.10703157014479722No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16680.10696744098353612No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16610.10651853685470833No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG16240.10414575788804716No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15940.10222188305021379No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15860.10170884976012488No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15750.10100342898625263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC18450.052.6703724
ATCAACG19350.050.2205923
CAACGCA22650.042.9036835
AACGCAG23750.040.9165656
ACGCAGA23850.039.839567
CGCAGAG24250.038.885588
GAATACG6050.033.3143779
TACATGG36100.033.299542
GTACATG37000.032.1396181
AATAGAC14700.030.8497923
ACATGGG36550.030.3292983
ATAGGTG13550.029.7493694
GCAGAGT32850.029.5819919
CAGCCGT15500.029.2575459
ACAGCCG15750.029.250178
ATAATAG16450.028.915891
GACCGAT1000.006291709428.7931397
GTCATAG14600.028.630811
TAATAGA16500.028.356882
TACACCG2405.6548743E-826.9935675
Produced by FastQC (version 0.11.5)