FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730868
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAG21600.29553900293897123No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG17650.24149367601263155No Hit
CTATAAATCAGTGCCGTGGGTTCTTGTCCAGGTTTCTGCTGGAACCAGTT16950.23191602313960935No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG16610.22726402031557No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT14600.19976247420874904No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC14130.193331764422577No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14120.19319494081010524No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG13900.19018482133572684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.1814281101375351No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCCGCACACC13260.1814281101375351No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGCCGTGGGTTCTTG13030.17828116705068492No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12180.16665115999058652No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11930.1632305696787929No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA11820.16172550994160367No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11040.15105326816880751No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA11040.15105326816880751No Hit
CTACTATGGTGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCG11030.15091644455633577No Hit
GTGTATTACTGTGCGAGACACAACTGGGGGGATGTCGGTCCTAGGGTTGA10600.14503302922005068No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10020.1370972596966894No Hit
TCCTCAGGCCGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG9960.13627631802185894No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9520.1302560790731021No Hit
GTAATACTCAGCCTCGTCCTCAGGCCGCACACCTGACAGTGTCAGGGCAG9510.13011925546063036No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG8870.12136254426243864No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8860.1212257206499669No Hit
TAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCCGCACACCTGA8840.1209520734250234No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8710.1191733664628907No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8590.11753148311322975No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA8410.11506865808873833No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8330.11397406918896437No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8270.11315312751413388No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8260.11301630390166213No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8080.11055347887717071No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7930.10850112469009451No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7770.10631194689054659No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7610.10412276909099864No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7350.10056535516673325No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGAGCACCACCA7340.1004285315542615No Hit
GTATTACTGCCTGCTCTACTATGGTGGTGGTGCTCAGGTGTTCGGCGGAG7330.10029170794178977No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7330.10029170794178977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG22500.055.6818773
TCAACGC22600.055.4317054
TATCAAC23800.053.2454952
GTATCAA24750.052.9726141
CAACGCA23650.052.970685
AACGCAG24200.051.76686
ACGCAGA25500.049.127717
CGCAGAG25800.048.8355188
GAATACG1452.0485459E-839.722769
GCAGAGT35850.035.145229
GAACCGT900.00375326731.998897
GTACATG21700.029.54511
GGTATCA14400.029.0148741
ATAGTGC1050.00797315727.427626
GTTATAC1856.133003E-627.2441623
GACTGAC1856.1362643E-627.2422987
CATGGGG10400.026.3067784
TATTGAG8100.025.7768845
CTAGAGA2808.818461E-925.7133944
CAGAGTA28900.024.6635069
Produced by FastQC (version 0.11.5)