FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730868
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTTATAGTACAAGCAACAAACACTCCTGGACCCCTGCCCGGTTCTCAG22530.3082635988988436No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG18820.25750203867182586No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG17550.2401254398879141No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15980.21864413272984998No Hit
CTATAAATCAGTGCCGTGGGTTCTTGTCCAGGTTTCTGCTGGAACCAGTT15320.20961377430671474No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT14740.20167800478335351No Hit
GTCCAGGAGTGTTTGTTGCTTGTACTATAAATCAGTGCCGTGGGTTCTTG14170.19387905887246398No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC13960.1910057630105573No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCCGCACACC13230.18101763930011985No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG12640.17294504616428685No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA12250.16760892527788876No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11310.15474750570554466No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11270.15420021125565764No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA11260.15406338764318592No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11220.15351609319329892No Hit
CTACTATGGTGGTGGTGCTCAGGTGTTCGGCGGAGGGACCAAGCTGACCG11110.15201103345610972No Hit
GTGTATTACTGTGCGAGACACAACTGGGGGGATGTCGGTCCTAGGGTTGA10670.14599079450735292No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10050.13750773053410464No Hit
GTAATACTCAGCCTCGTCCTCAGGCCGCACACCTGACAGTGTCAGGGCAG9280.1269723123737802No Hit
TCCTCAGGCCGCACACCTGACAGTGTCAGGGCAGCTTTGCCCCCAAGGAG9100.12450948734928878No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8810.12054160258760815No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8770.11999430813772118No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8650.11835242478806021No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAG8280.11328995112660563No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCA8160.11164806777694466No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8060.11027983165222723No Hit
TAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCCGCACACCTGA7970.1090484191399815No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7540.10316500380369642No Hit
ATCCAAACTGGTTCCAGCAGAAACCTGGACAAGAACCCACGGCACTGATT7530.10302818019122467No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7320.10015488432931802No Hit
GTATTACTGCCTGCTCTACTATGGTGGTGGTGCTCAGGTGTTCGGCGGAG7310.10001806071684627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)