FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700665
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTCAGTGGACGTTCGGCCAAGGGACCAAGGTAGAAGTCAAAC23440.33453933049317436No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21960.3134165400012845No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCAAAGAGTTCAATATCAGGGAGA21080.30085704295205273No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCT19960.28487222852575766No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16220.23149436606652252No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA16030.2287826564763475No Hit
TCTATAAGGCGTCCAGTTTAAAAAGTGGGGCCCCATCAAGGTTCAGTGGC15160.2163658809844933No Hit
GTCTCTGGTGGCTCTGTCAACAGGGAAAGTGACTACTGGGTCTGGATCCG14340.20466271327952731No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCGTCAGGCTGCAGGCTGC14230.20309277614837334No Hit
GTGTATTACTGTGCGAGAGATCTCGCCAGTCTCTTTGACTACTGGGGCCA13970.19938201565655486No Hit
ATAATAGTCAGTGGACGTTCGGCCAAGGGACCAAGGTAGAAGTCAAACGA13900.1983829647549114No Hit
CAGTATAATAGTCAGTGGACGTTCGGCCAAGGGACCAAGGTAGAAGTCAA13760.19638486295162455No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13740.19609941983686927No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11620.1658424496728108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11500.1641297909842792No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11110.15856365024655147No Hit
GTAGGAGACAGAGTCACCATCGCTTGCCGGACCAGTCAGAGTATTAATAA10660.15214118016455797No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10320.1472886472137184No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10240.14614687475469731No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10120.14443421606616572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.14043801245959195No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9240.13187471901693393No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9080.12959117409889176No Hit
GACTATTATACTGTTGGCAGTAATAAGTTGCAAAATCGTCAGGCTGCAGG8980.12816395852511542No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8790.12545224893494036No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT8440.12045699442672318No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8320.11874433573819158No Hit
ATCTGGGACAGACTTCAATCTCACCATCAGCAGCCTGCAGCCTGACGATT8310.11860161418081394No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7950.1134636381152191No Hit
CACCTGCACTGTCTCTGGTGGCTCTGTCAACAGGGAAAGTGACTACTGGG7860.1121791440988204No Hit
GTATTACTGTGCGAGAGATCTCGCCAGTCTCTTTGACTACTGGGGCCAGG7720.11018104229553352No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7150.1020459135250084No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC7120.10161774885287549No Hit
CCTTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAG7050.10061869795123204No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7040.10047597639385442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.084.621571
ATCAACG15550.062.0382273
TCAACGC15550.062.0382274
GGTATCA5350.062.028131
TATCAAC16000.059.88192
CAACGCA17600.054.812185
AACGCAG17850.054.0445026
ACGCAGA19350.049.8550037
CGCAGAG19500.049.4715088
GCAGAGT23550.041.2693639
GTATTAG2103.9835868E-1034.3530541
GTTACAC1905.806214E-934.1015323
TACACCG1102.5380094E-432.723695
GTATATA3650.027.6706771
GTACATG15400.026.233241
AGCTAGC1100.00997540126.1957742
TCTATAC1658.827135E-526.1789533
TACACGG10200.026.1147865
ATACACG10700.025.5672934
GTTGTAC1701.066182E-425.4616741
Produced by FastQC (version 0.11.5)