FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855178
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACCGGAGCCCACTGATGTCCAGGGAGG33710.39418694119820674No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG26010.3041472067803428No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20380.2383129594072813No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20210.2363250691668869No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16750.19586565603885975No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG15910.18604313955691096No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC14570.1703738870738022No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14510.16967227875366298No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG13590.1589142845115286No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA13510.157978806751343No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12800.14967644162969582No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12290.14371277090851262No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11900.1391523168276078No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11860.13868457794751501No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC11620.13587814466695822No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11570.13529347106684222No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11550.1350596016267958No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11440.13377331970654063No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11030.12897899618558945No Hit
ACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG10860.12699110594519503No Hit
GTTTAGGGGCCGTTCCCGGGAGCTGCTGGTACCAATTTACACTATTACTT10620.12418467266463824No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10580.12371693378454543No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10430.12196291298419745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10290.12032582690387264No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10190.11915647970364066No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10080.11787019778338545No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9950.11635004642308384No Hit
TTATTATTATAGATGAGGAGTTTAGGGGCCGTTCCCGGGAGCTGCTGGTA9840.11506376450282865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9600.11225733122227186No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9220.10781381186139025No Hit
CATTATTATTATAGATGAGGAGTTTAGGGGCCGTTCCCGGGAGCTGCTGG9070.10605979106104227No Hit
GTACATGGGAGCTTCAGCTGTGCGTAGAGAAGACAGGACTCAGGACAATC8930.10442270498071747No Hit
ATAATAATGAGCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG8830.10325335778048547No Hit
ATGTATTACTGTGCGAGACCCCAAAATTACGGTGATAACTCGGGGGTGGT8780.10266868418036947No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGGC8710.10185014114020706No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8690.10161627170016066No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8680.10149933698013747No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8670.10138240226011427No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8630.10091466338002146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)