FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855178
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG25340.2963125805387884No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21580.2523451258100653No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20060.2345710483665389No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC18460.2158614931628269No Hit
GTAATAATCAGCCTCATCCTCAGACCGGAGCCCACTGATGTCCAGGGAGG17210.20124465315992696No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT17170.20077691427983413No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15670.18323670627635416No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15290.17879318691547255No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG15100.17657142723503177No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG13670.15984976227171419No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC12840.1501441805097886No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12760.149208702749603No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12540.1466361389090926No Hit
ACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12330.14418050978860542No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12160.142192619548211No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11840.1384507085074686No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA11080.1295636697857054No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11050.12921286562563583No Hit
ATAATAATGAGCGACCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11010.12874512674554303No Hit
TTATTATTATAGATGAGGAGTTTAGGGGCCGTTCCCGGGAGCTGCTGGTA10900.12745884482528783No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10800.12628949762505584No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10750.12570482402493985Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC10700.12512015042482383No Hit
GTTTAGGGGCCGTTCCCGGGAGCTGCTGGTACCAATTTACACTATTACTT10630.12430160738466144No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10470.12243065186429024No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10290.12032582690387264No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10190.11915647970364066No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9610.11237426594229505No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9580.11202346178222547No Hit
GTACATGGGAGCTTCAGCTGTGCGTAGAGAAGACAGGACTCAGGACAATC9050.10582592162099586No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9010.10535818274090307No Hit
CATTATTATTATAGATGAGGAGTTTAGGGGCCGTTCCCGGGAGCTGCTGG8930.10442270498071747No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8800.10290255362041587No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8720.10196707586023025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8650.10114853282006787No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)