FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732555
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22410.30591559678112906No Hit
CTACTAAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG19670.26851226187794774No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17470.23848038713816708No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16950.23138194401785533No Hit
CTACTGGGTCTGGCTCCGCCAGCCCCCCGGGAAGGGGCTGCAGTGGATTG15630.21336281917398695No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15370.20981359761383106No Hit
GAAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGTTTCAGGGAGA14450.19725481363174097No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC13200.18019124843868378No Hit
ATTACAACCCGTCCCTCCAGAGTCGAGTCATCGTATCTGTAGACACGTCC12940.1766420268785279No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12780.17445789053381655No Hit
GTGTAAGTTCGTCTCGCACAGAAATAGACAGCCGTGTCTGCGGCGGTCAC12710.17350233088300537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12490.1704991434090273No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11950.1631276832456266No Hit
TTATTACTGCATGCAAGCTCTACTAAACCCGCTCACTTTCGGCGGAGGGA11900.1624451406379043No Hit
GTCTATTTCTGTGCGAGACGAACTTACACTGGTAACTCTCCCCCTCGCTG11760.16053402133628192No Hit
GTAATAAACTCCAACATCTTCAGCCTCCACTCTACTGATTTTCAGTGTAA11740.160261004293193No Hit
GGTTTAGTAGAGCTTGCATGCAGTAATAAACTCCAACATCTTCAGCCTCC11380.15534669751759253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11380.15534669751759253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11340.1548006634314147No Hit
GGCCAGGGCAGTCTCCACAACTCCTGATCTACTCGGGCTCTATTCGGGCC10950.14947683109118087No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10750.1467466606602917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10500.14333394762168028No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10450.142651405013958No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10390.14183235388469126No Hit
AACTTACACTGGTAACTCTCCCCCTCGCTGGGGCCAGGGAACCCTGGTCA10050.1371910641521797No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA9990.13637201302291294No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9920.13541645337210176No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG9690.13227675737657923No Hit
CAGTAATAAACTCCAACATCTTCAGCCTCCACTCTACTGATTTTCAGTGT9550.13036563807495682No Hit
CTGTAGACACGTCCAAGAACCAATTCTCCCTGAAACTGAACTCTGTGACC9400.12831801025178996No Hit
GGATTGGGAGTATCTATTTTGGTGGGAACACCTATTACAACCCGTCCCTC9190.12545133129935637No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA9140.12476878869163406No Hit
GTACGTGCAGAGGCCAGGGCAGTCTCCACAACTCCTGATCTACTCGGGCT9060.12367672051927842No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC9040.12340370347618951No Hit
GTTTATTACTGCATGCAAGCTCTACTAAACCCGCTCACTTTCGGCGGAGG8680.11848939670058903No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCGGAGCCTCCTG8650.11807987113595567No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8630.11780685409286676No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8530.11644176887742218No Hit
GTAATAGGTGTTCCCACCAAAATAGATACTCCCAATCCACTGCAGCCCCT8340.11384810696807748No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG8280.11302905583881073No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8190.11180047914491062No Hit
GCCCTGGCCTCTGCACGTACCACTCCAAAAAGTTGAATCCATAACCAGAC8030.1096163428001993No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT7860.10729569793394352No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7760.10593061271849896No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7660.10456552750305438No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC7630.10415600193842102No Hit
CTACAAGACCACACCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT7470.10197186559370969No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7430.10142583150753186No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA7400.10101630594289848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12650.060.903583
TATCAAC13100.059.361122
GTATCAA13500.059.246821
TCAACGC13450.057.8124544
CAACGCA13950.055.736525
AACGCAG14350.054.684586
TAATACG450.00990636547.9986154
ACGCAGA16400.047.845747
CGCAGAG16550.047.4120948
GCAGAGT19450.040.342949
GGTATCA5600.037.3152051
GTACATG18100.034.635111
TAGACTA1051.9312468E-434.2823835
GTGCTAG2551.8189894E-1233.909151
TACATGG18050.033.907152
CAGAGTA18050.033.9002089
ACATGGG18750.033.02533
TATAGAC1102.5359035E-432.728563
ATAGTCC1153.2954031E-431.299178
GAATACG2101.5414116E-830.852049
Produced by FastQC (version 0.11.5)