FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732555
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21660.2956774576652948No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18960.2588201568482913No Hit
CTACTAAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG18300.24981059442635706No Hit
CTACTGGGTCTGGCTCCGCCAGCCCCCCGGGAAGGGGCTGCAGTGGATTG15970.21800410890649846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15830.2160929896048761No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15540.2121342424800868No Hit
GAAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGTTTCAGGGAGA13610.18578809782200653No Hit
GTGTAAGTTCGTCTCGCACAGAAATAGACAGCCGTGTCTGCGGCGGTCAC12700.17336582236146092No Hit
ATTACAACCCGTCCCTCCAGAGTCGAGTCATCGTATCTGTAGACACGTCC12650.17268327975373865No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC12410.16940707523667164No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12050.1644927684610712No Hit
TTATTACTGCATGCAAGCTCTACTAAACCCGCTCACTTTCGGCGGAGGGA11970.1634007002887155No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11650.15903242759929287No Hit
GGTTTAGTAGAGCTTGCATGCAGTAATAAACTCCAACATCTTCAGCCTCC11350.15493717195295917No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11300.15425462934523687No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11250.15357208673751457No Hit
GGCCAGGGCAGTCTCCACAACTCCTGATCTACTCGGGCTCTATTCGGGCC10950.14947683109118087No Hit
GTCTATTTCTGTGCGAGACGAACTTACACTGGTAACTCTCCCCCTCGCTG10950.14947683109118087No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10690.14592760953102496No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10650.14538157544484714No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT10580.14442601579403594No Hit
GTAATAAACTCCAACATCTTCAGCCTCCACTCTACTGATTTTCAGTGTAA10470.14292442205704692No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10420.14224187944932462No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA9950.13582597893673512No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG9940.13568947041519067No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA9930.1355529618936462No Hit
AACTTACACTGGTAACTCTCCCCCTCGCTGGGGCCAGGGAACCCTGGTCA9830.13418787667820162No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9680.13214024885503478No Hit
CAGTAATAAACTCCAACATCTTCAGCCTCCACTCTACTGATTTTCAGTGT9530.13009262103186792No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9420.1285910272948789No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9280.12667990799325649No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT9270.126543399471712No Hit
GTACGTGCAGAGGCCAGGGCAGTCTCCACAACTCCTGATCTACTCGGGCT8940.12203861826074493No Hit
GGATTGGGAGTATCTATTTTGGTGGGAACACCTATTACAACCCGTCCCTC8900.1214925841745671No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCGGAGCCTCCTG8760.1195814648729447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8720.11903543078676687No Hit
CTGTAGACACGTCCAAGAACCAATTCTCCCTGAAACTGAACTCTGTGACC8710.1188989222652224No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8360.11412112401116639No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8350.11398461548962195No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8310.11343858140344411No Hit
GTTTATTACTGCATGCAAGCTCTACTAAACCCGCTCACTTTCGGCGGAGG8120.11084491949409941No Hit
GCCCTGGCCTCTGCACGTACCACTCCAAAAAGTTGAATCCATAACCAGAC7740.10565759567541004No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7630.10415600193842102No Hit
GTAATAGGTGTTCCCACCAAAATAGATACTCCCAATCCACTGCAGCCCCT7600.10374647637378764No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC7580.10347345933069871No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7500.10238139115834306No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC7410.10115281446444295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9600.054.7653243
TCAACGC9700.053.4582564
TATCAAC9850.053.3862842
CAACGCA10500.050.0711565
GTATCAA10650.050.052431
AACGCAG10850.048.4460266
CGCAGAG11550.045.5067948
ACGCAGA12500.042.0511477
AGTGGTG6400.037.135395
ACATTAG4850.037.1161847
GCAGAGT14350.036.627429
GTACATG17500.033.7535061
GGTATCA4950.033.4707681
GGTGGGT6550.032.9773188
TACATGG17800.032.7799342
GAATACG1801.3723911E-732.0002149
ACATGGG18550.031.8364033
GTATTGC1153.2828585E-431.319531
TATAAGG1402.8690753E-530.8721072
GTGGGTG7300.030.5755469
Produced by FastQC (version 0.11.5)