FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940223
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTAGG24460.2601510492723535No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16750.17814922630056912No Hit
GTTTATTACTGTGCGCGGGGGGATTATTATGCTTCAGGGAGACGATTCGA16440.17485213614217054No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA12390.1317772485888986No Hit
TTATTACTGTGCGCGGGGGGATTATTATGCTTCAGGGAGACGATTCGACC11810.12560849926028186No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCACCGAAGTGTCG11550.12284319783710887No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10910.11603630202622144No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAAGACACGGCTGTTTATTA10580.11252649637373262No Hit
CAGTAATAAACAGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTA10340.10997391044464983No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10200.1084849019860182No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9610.10220979491035638No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGCAC9520.10125257518695033No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9490.10093350194581498No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG450.00989572248.0127335
TCAACGC13650.046.432374
CAACGCA14950.042.3925175
AACGCAG15300.041.413946
TATCAAC15850.039.9874952
ACGCAGA16300.038.8732077
CGCAGAG17050.037.585558
GTATCAA18700.037.367451
ACAGCCG12650.035.859598
CAGCCGT12400.034.259869
TCTACAC9350.030.0416553
GTACATG24000.029.4156281
ATCAACG25350.025.854393
GTGCTAG4200.025.7279571
TATTGAG12350.025.0754785
CCCTATA2651.466833E-724.4606232
GTAATAA21500.024.1244471
GAATACG3001.835906E-824.0012599
CTAGGTC11700.023.3920653
ACATGGG28850.022.9674553
Produced by FastQC (version 0.11.5)