FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648970
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC43630.2645894103591939No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGACCAGAGATGGTCAGGGAGG35250.21376980781942664No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA33350.2022474635681668No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30160.1829020540094726No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT29860.18108273649611575No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT29710.18017307773943733No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGTCTGCCTCCGTGTCTGGGTCTC29600.1795059946512065No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGGTACTA28550.1731383833544576No Hit
GATTATTACTGCACCTCATATACAAGTAGTACCACTTGGGTGTTCGGCGG27810.16865073348817747No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT26920.16325342486521888No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25280.15330782245886826No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAATTATGTCTCCT24250.14706149899634316No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA24010.1456060449856577No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT22900.13887457018623747No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22750.13796491142955905No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22670.1374797600926639No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG22410.13590301824775466No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22180.1345082081541811No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21800.13220373930392912No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21740.13183987580125775No Hit
CCTCATATACAAGTAGTACCACTTGGGTGTTCGGCGGAGGGACCAAGCTG21660.1313547244643626No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT21490.1303237778734604No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21120.12807995294032032No Hit
GCTATATATTTTTGTGCGAGAGATAGCCACGCGCTGGGGAAGGCTTTTGA20240.12274328823447364No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19920.12080268288689304No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG19480.11813435053396967No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19320.1171640478601794No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT18990.11516279859548689No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA18850.11431378375592037No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18830.11419249592169657No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGTCT18310.11103901223187809No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT18100.1097654899725283No Hit
CTACAAGACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT17940.10879518729873801No Hit
GTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGACCAGA17830.10812810421050717No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17770.1077642407078358No Hit
TATACAAGTAGTACCACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT17760.1077035967907239No Hit
TGTCAGTACTCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGT17410.1055810596918076No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC17290.10485333268646488No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17240.10455011310090542No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG17080.1035798104271151No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17000.10309465909021996No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG16990.10303401517310806No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16700.10127534157686313No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG16660.10103276590841556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)