FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648970
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36760.2229270393033227No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGACCAGAGATGGTCAGGGAGG30950.18769292346131222No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT30040.18217432700412986No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA29360.17805054064052103No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGTCTGCCTCCGTGTCTGGGTCTC26270.1593115702529458No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT25960.15743160882247706No Hit
GATTATTACTGCACCTCATATACAAGTAGTACCACTTGGGTGTTCGGCGG25000.15160979277973524No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24640.14942661176370703No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23480.14239191737872733No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGGTACTA23380.1417854782076084No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA21400.12977798261945336No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21380.12965669478522956No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19580.11874078970508864No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAATTATGTCTCCT19370.11746726744573885No Hit
CCTCATATACAAGTAGTACCACTTGGGTGTTCGGCGGAGGGACCAAGCTG19300.1170427600259556No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19090.11576923776660583No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18950.11492022292703931No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17970.10897711905007368No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17830.10812810421050717No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT17630.10691522586826928No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17510.10618749886292654No Hit
GTATATGAGGTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGACCAGA17500.10612685494581466No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17070.10351916651000322No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA16780.1017604929137583No Hit
GCTATATATTTTTGTGCGAGAGATAGCCACGCGCTGGGGAAGGCTTTTGA16710.10133598549397502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)