FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867088
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA40990.47273171811857617No Hit
ATTTATTACTGTGCGAATATAGGATATTGTACTGGTGGTGTGTGCTCTGA39140.45139593674459805No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG21480.2477257210340819No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21290.24553447862270036No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17050.19663517428450167No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16210.18694757625523592No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15590.17979720628125404No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT15400.17760596386987249No Hit
ATATTGTACTGGTGGTGTGTGCTCTGACCACCACTCCCCCTACCACTATT15330.17679866403410033No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15220.17553005000645838No Hit
CCGTAATAGTGGTAGGGGGAGTGGTGGTCAGAGCACACACCACCAGTACA15010.17310815049914197No Hit
GTACAATATCCTATATTCGCACAGTAATAAATAGCCGTGTCCTCAGCTCT14560.1679183658406067No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14440.16653442326499734No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13700.15800011071540604No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13640.15730813942760136No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12160.1402395143284188No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12080.1393168859446792No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12020.1386249146568745No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTTTGCTATGCACTGGG11740.1353957153137859No Hit
GTACTGGTGGTGTGTGCTCTGACCACCACTCCCCCTACCACTATTACGGT11680.13470374402598123No Hit
ATATTACTGTCAGCAGTATGCCACTCTCCCTCCGTGGACGTTCGGCCAAG11570.13343512999833926No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11310.13043658775118558No Hit
GATTTCATATGATGGAAGTAAAAAGTATAACGCAGACTCCGTGAAGGGCC10900.12570811728452014No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10700.12340154632517115No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10570.1219022752015943No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10130.11682781909102653No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9860.11371394829590538No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9850.11359861974793793No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9720.11209934862436108No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9660.11140737733655638No Hit
CCACCAGTACAATATCCTATATTCGCACAGTAATAAATAGCCGTGTCCTC9450.10898547782923994No Hit
ATCCTATATTCGCACAGTAATAAATAGCCGTGTCCTCAGCTCTCAGGCTG9340.10771686380159799No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTTTGCT9190.10598693558208626No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9120.10517963574631409No Hit
GTATAACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT9040.10425700736257451No Hit
GGGCTGGAGTGGCTGGCACTGATTTCATATGATGGAAGTAAAAAGTATAA8840.10195043640322551No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC8840.10195043640322551No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC8450.048.5791974
ATCAACG9000.045.6104743
TATCAAC9600.045.012932
CAACGCA9150.044.8601725
GTATCAA9900.043.6589851
CTGTGCG16550.041.3292549
GGCAGCG4100.038.6318667
TACATGG17350.038.6047552
TATATCG951.0704764E-437.901255
GTACATG17350.037.7832641
CGCAGAG10800.037.333298
ACTGTGC20450.036.968178
ACATGGG17800.036.4128573
GCAGCGC4500.035.199968
GAATACG2104.0745363E-1034.285679
TACTGTG20550.033.6330577
TTATTAC22600.032.184383
TACACGG8100.031.9981166
GTACACG8250.031.4235825
CAGCGCT4750.030.3157549
Produced by FastQC (version 0.11.5)