FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649415
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGTTTTTATTGGGCCTGGATCCGGCA23570.36294203244458473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19290.29703656367653963No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAATTTCAGGAAGA18950.291801082512723No Hit
GTTATAACCACTCCAAAATTCGTAATATTTATTTCTCGCACAGTAATACA16440.25315091274454704No Hit
GGTTATAACATCGAAAAGGGGTGGTTCGACCCCTGGGGCCAGGGAACCCA15700.24175604197624015No Hit
GTGTATTACTGTGCGAGAAATAAATATTACGAATTTTGGAGTGGTTATAA13010.2003341468860436No Hit
ACCTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTGTGATTTTCGG12240.18847732189739996No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10540.16229991607831665No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAACTTCTGATGTACACT10090.15537060277326517No Hit
ATCTATGATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGT10050.15475466381281616No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9230.14212791512361125No Hit
GTCATAGGACTGGCAGTAATAGGTAGCCTCATCCTCACTCTGGAGCCCAG9130.1405880677224887No Hit
TCCTATGACAGCAGCCTGAGTGGTGTGATTTTCGGCGGAGGGACGAAGCT9090.13997212876203968No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.13935618980159067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.13889423558125388No Hit
GTAATAGGTAGCCTCATCCTCACTCTGGAGCCCAGTGATGGCCAGGGAGG8850.13627649499934558No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8760.13489063233833526No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8310.1279613190332838No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8160.1256515479316No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8130.1251895937112632No Hit
GTATTACTGTGCGAGAAATAAATATTACGAATTTTGGAGTGGTTATAACA8110.12488162423103871No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7870.12118599046834458No Hit
GATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAACTCCTCAT7720.11887621936666076No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGTTTTTATTGGGCCT7600.11702840248531371No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7580.1167204330050892No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7540.11610449404464017No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7450.1147186313836299No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA7430.11441066190340538No Hit
GACGTAGGACGGTCAGCTTCGTCCCTCCGCCGAAAATCACACCACTCAGG7320.11271682976217057No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7260.11179292132149704No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7220.11117698236104802No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7170.11040705866048675No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7010.10794330281869066No Hit
ATCATAGATGCTCCCAATCCACTCCAGACCCCTCTCTGGCGTCTGCCGGA6970.10732736385824165No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6960.1071733791181294No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6940.10686540963790489No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA6930.10671142489779263No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6770.10424766905599654No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6770.10424766905599654No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC6660.10255383691476175No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG6600.10162992847408822No Hit
CTATGATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGTAA6590.10147594373397598No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGATAACAACCATCGGCCCT6590.10147594373397598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6650.084.6190641
GTATCAA17650.071.938811
ATCAACG21600.057.7189673
TCAACGC21750.057.652244
TATCAAC22400.055.9792982
CAACGCA23600.052.827535
AACGCAG24300.051.534776
ACGCAGA26900.046.550137
CGCAGAG27050.046.2919968
GCAGAGT30550.040.9884959
TAACGCA750.001527478738.4348344
TATCACG800.002097081236.0326542
CAGAGTA30650.034.9848259
GTATAGA1957.3287083E-933.2968521
GAATACG1801.3778663E-731.9846349
GTACATG20050.028.7853091
TATTGAG8100.028.4702435
GTATTAG1804.880383E-628.055681
TACATGG20450.027.4870112
TTACTCC14400.027.4867959
Produced by FastQC (version 0.11.5)