FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649415
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGTTTTTATTGGGCCTGGATCCGGCA21840.33630267240516465No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAATTTCAGGAAGA19160.29503476205508034No Hit
GTTATAACCACTCCAAAATTCGTAATATTTATTTCTCGCACAGTAATACA17600.2710131425975686No Hit
GGTTATAACATCGAAAAGGGGTGGTTCGACCCCTGGGGCCAGGGAACCCA15880.24452776729826076No Hit
GTGTATTACTGTGCGAGAAATAAATATTACGAATTTTGGAGTGGTTATAA14050.21634855985771811No Hit
ACCTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTGTGATTTTCGG11080.17061509204437839No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAATGTCTGGGGCCC10490.16152999237775537No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAACTTCTGATGTACACT9740.14998113686933626No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9320.14351377778462154No Hit
ATCTATGATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGT8900.13704641869990683No Hit
TCCTATGACAGCAGCCTGAGTGGTGTGATTTTCGGCGGAGGGACGAAGCT8590.13227289175642692No Hit
GTATTACTGTGCGAGAAATAAATATTACGAATTTTGGAGTGGTTATAACA8230.12672944111238577No Hit
ATCATAGATGCTCCCAATCCACTCCAGACCCCTCTCTGGCGTCTGCCGGA7940.12226388364913038No Hit
GATGTACACTGGTACCAACAACTTCCAGGAACAGCCCCCAAACTCCTCAT7840.12072403624800782No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG7820.12041606676778331No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7630.11749035670565047No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7420.11425667716329312No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7400.11394870768306861No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGTTTTTATTGGGCCT7270.1119469060616093No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7200.11086901288082351No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7170.11040705866048675No Hit
CTATGATAGTGGGAGCACTTACTACAACCCGTCCCTCAAGAGTCGAGTAA6800.1047096232763333No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAATTTCAGGAA6540.10070602003341468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)