FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798773
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATGTCAGGCTGTTCAACTCCATGGAGG22330.2795537655879705No Hit
GTCTATTACTGTGCGAGAGAGGATGGAGTACACCCGTCTGGGTATTACTT17220.21558064681705566No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12050.15085637596663884No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11280.14121659094636396No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11040.13821198262835624No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10880.13620891041635108No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10530.13182718995258977No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10030.12556758929007367No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9190.11505146017704655No Hit
TCCCTATGTTTGGCACCGCAAACTACGCACAGAAATTCCAGGGCAGAGTC8900.11142089179278719No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8730.10929262756753169No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8620.10791551542177816No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA8560.1071643633422762No Hit
GAGTACACCCGTCTGGGTATTACTTTGACCTCTGGGGCCAAGGAACCCTG8540.10691397931577556No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8010.10027880261350847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.064.240131
TCAACGC15700.053.1897284
ATCAACG16150.051.707663
CAACGCA16400.050.9194345
AACGCAG17350.048.131346
TATCAAC17950.046.5399742
CGCAGAG20550.040.9867488
ACGCAGA22850.036.5461167
CTACGTT900.00375550631.9953544
TACACGG6150.031.6051656
GTACACG6450.030.1351595
TAGACGG11550.029.9177345
ACCGGGC1453.661923E-529.7887788
ATAGACG11950.028.9163044
AGACGGC12550.027.533856
ACACGGG7150.027.1848667
GGTATCA13400.026.9156861
GTATTAG4300.026.8405721
TAGACTG3006.366463E-1026.3961685
GCAGAGT32450.026.1780179
Produced by FastQC (version 0.11.5)