FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962505
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14340.1489862390325245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC31450.052.192454
GTATCAA32250.052.141241
ATCAACG31500.051.8810543
CAACGCA32400.050.6621135
TATCAAC33800.048.3909072
AACGCAG34800.047.3750576
CGCAGAG36950.044.423618
ACGCAGA39750.041.4755177
TAGACCG700.001093696641.139165
GCAGAGT43500.037.7345439
GGTATCA18600.036.0848661
GACGTAT1000.00621134628.8678951
GTACATG35300.028.0092221
TACACCG2604.0090526E-927.689825
ACATGGG35000.027.3575423
CAGAGTA42850.026.7140549
TACATGG40000.024.3180312
TATACAG7750.023.2237195
GTACACG3956.4392225E-1021.8714525
TACACGG4201.3842509E-920.569586
Produced by FastQC (version 0.11.5)