FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962505
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG16500.051.043683
TCAACGC16500.051.043684
TATCAAC18650.046.317222
GTATCAA20800.042.6121331
CGCAGAG19700.042.021518
CAACGCA21350.039.785445
AACGCAG21800.038.3037726
ACGCAGA22050.037.869497
GTACATG32050.035.0742261
ACATGGG31200.033.9158553
GCAGAGT26150.031.9320169
TACATGG35650.031.2976842
TATTACG1050.00798132827.4227337
GGTATCA13950.026.3443491
CATGGGG16100.024.5910364
GTATATA9150.022.8385451
CATGGGA24000.022.7951474
CCGTGCA6200.022.059829
GTACACG4609.094947E-1221.9083795
GAATACG2303.3196928E-521.9083799
Produced by FastQC (version 0.11.5)