FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829550
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATTCA39320.47399192333192697No Hit
GTGTATTACTGTGCGAGAGATGCCGCCTCCTGGACAACTGGAACTTCCGT27260.3286118980169972No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24530.2957024893014285No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGTGTTGCTG16680.20107287083358447No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTTTCCCCAGGACAGACA15420.18588391296486048No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGATACG15260.18395515641010185No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14260.1719004279428606No Hit
CCCCTGTGTTGGTCATTTATGAAGATGACAAGCGGCCCTCAGGGATCCCT14200.1711771442348261No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14190.1710565969501537No Hit
GTATTTATTTCTTCCATCATATGATATAACTGCCACCCATTCCAGCCCCT14150.17057440781146405No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13610.16406485443915378No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGC13060.15743475378217106No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAATT13060.15743475378217106No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12850.1549032608040504No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12540.15116629497920558No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAATGGGTGGC12160.14658549816165392No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11940.14393345789886083No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11830.1426074377674643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11830.1426074377674643No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11640.14031703935868844No Hit
AATAAATACTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA11640.14031703935868844No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11390.13730335724187812No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10900.13139654029292988No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10780.12994997287686094No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10380.12512808148996443No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10200.12295823036586101No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10200.12295823036586101No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATT10060.12127056838044724No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10040.1210294738111024No Hit
GTATTACTGTGCGAGAGATGCCGCCTCCTGGACAACTGGAACTTCCGTTT9850.11873907540232656No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9850.11873907540232656No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9790.11801579169429209No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATTCAGCG9670.11656922427822312No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9630.11608703513953347No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9560.1152432041468266No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9500.11451992043879212No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9500.11451992043879212No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9310.11222952203001628No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCATCC9220.11114459646796455No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9090.10957748176722319No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9030.10885419805918871No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9010.10861310348984389No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8890.10716653607377494No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGCAGTGTTGCTGT8770.105719968657706No Hit
GTGTTGGTCATTTATGAAGATGACAAGCGGCCCTCAGGGATCCCTGAGCG8690.10475559038032667No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8660.10439394852630945No Hit
ACACAGGGGACTGGCCTGGCTTCTGCTGATACCAGCAAACATACTGATCC8660.10439394852630945No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGGGGGACAGCAACACTG8510.10258573925622325No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8410.10138026640949913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21450.052.1665571
ATCAACG23050.049.0355073
TCAACGC23150.048.8236924
CAACGCA24350.046.713255
TATCAAC24100.046.3519552
AACGCAG24700.045.7598536
GAATACG3100.041.8015869
ACGCAGA28050.040.2947777
CGCAGAG28500.039.405948
GCAGAGT30700.036.5820669
TACACCG1251.3272798E-534.555985
ACAGCCG17500.034.14468
TACATGG24250.033.285852
GTACATG24250.033.0444831
CAGCCGT17900.032.9794049
GTAATAC20100.031.9655151
AATACAC19600.031.588163
GCTAGAG4650.030.9641363
GTGCTAG4550.030.1460191
GTATAGA2401.6552804E-930.0799081
Produced by FastQC (version 0.11.5)