FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences945852
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT58660.6201815928919112No Hit
TTATAATACTCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC49960.5282010293365136No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44410.4695237732753116No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA38450.4065118010005793No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG35320.3734199430777754No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32410.3426540304402803No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28190.29803817087662765No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23460.24803034724248615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22570.23862084131555467No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22490.23777504302998778No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22210.2348147490305037No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT21800.2304800328169735No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20230.21388124146272358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19560.20679768082110098No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA19390.2050003594642714No Hit
ATATTATAATACTCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCA18320.1936878073948144No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17730.1874500450387587No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16980.17952068611156927No Hit
TTATTACTGTCAGCAATATTATAATACTCCTCCCACTTTTGGCCAGGGGA16640.17592604339791004No Hit
ATTATAATACTCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA16560.17508024511234316No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16490.17434017161247214No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA16270.17201422632716323No Hit
ATAATACTCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA16000.16915965711337505No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15800.16704516139945783No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA15770.16672798704237027No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG15170.1603844999006187No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCTCCCACTTTTGGCCAGGG14990.15848145375809325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC14950.1580585546153098No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14620.15456963668734644No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTA14590.15425246233025885No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14580.154146737544563No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14450.15277231533051683No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14110.1491776726168576No Hit
GTAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATTGTAAGGACCA13790.14579447947459012No Hit
TCCTCGTACTGTGTGCACAGTAATATGTGGCTGTGTCCACAGGGTCCATG13780.14568875468889422No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC12960.13701932226183378No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT12900.1363849735476586No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12600.13321322997678284No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC12580.1330017804053911No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12530.13247315647691182No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12320.13025293597729878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.12951286247742777No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG12220.12919568812034019No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12120.1281384402633816No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12050.12739836676351057No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11990.12676401804933543No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA11870.12549532062098512No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11750.12422662319263478No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA11710.12380372404985135No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11630.12295792576428448No Hit
GCAATATTATAATACTCCTCCCACTTTTGGCCAGGGGACCAAGCTGGAGA11620.12285220097858862No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA11510.12168922833593417No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11210.11851748476505838No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT11130.11767168647949151No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11100.11735451212240393No Hit
GAGTTACAGTACCCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCA11100.11735451212240393No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT11030.11661443862253291No Hit
GTACGAGGAATAACTGGAACTACGGCTACTGGGGCCAGGGAACCCTGGTC11010.1164029890511412No Hit
ATCCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT10860.11481711726570332No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG10780.11397131898013642No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10230.10815645576686417No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10080.10657058398142627No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT10010.10583051048155526No Hit
TACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT9960.10530188655307597No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA9950.10519616176738009No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG9950.10519616176738009No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9880.10445608826750907No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9820.10382173955333393No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC9750.10308166605346292No Hit
GCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAG9750.10308166605346292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23750.060.4652371
ATCAACG23500.059.7377243
CAACGCA24100.058.250485
TCAACGC24300.057.7710574
AACGCAG24800.057.1868936
TATCAAC24450.057.1735652
GTATACG400.006163239554.1224631
ACGCAGA28150.049.614127
CGCAGAG28600.048.833488
GAATACG2150.043.5298169
GCAGAGT32600.042.8416379
GTGCTAG2950.041.585621
GTGCGGA1001.430014E-436.0816421
ATAGGTG7850.035.766544
GTCATAG7850.034.932551
TCATAGG8450.034.1095282
TAGGTGG8400.032.567645
GGTATCA24000.032.4734761
CAGAGCG8900.032.3557859
ACTATAC1352.2476555E-531.9962773
Produced by FastQC (version 0.11.5)