FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907777
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGCAGAGACA14930.1644677051742884No Hit
GTGTATTACTGTGCAACAGGAGACAGCTATGGTTATAGTTACTGGGGCCA10440.1150062184875801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.044.6070181
TCAACGC10550.044.349774
ATCAACG10750.042.855053
CAACGCA10950.042.072315
AACGCAG11950.037.949246
TATCAAC13000.035.9915432
GGTATCA6450.033.5266721
ACGCAGA13850.032.743217
CGCAGAG14950.029.852528
GTACATG21900.029.6228851
TACATGG21450.026.846842
ACATGGG22450.025.650993
GTAATAC13300.025.472711
TAGTACC3102.6078851E-823.2203524
AATACAC14350.023.0747183
GCAGAGT19350.023.064359
TACACAG19400.023.0049065
TAGACTA2552.8801333E-622.582935
TAGGACG7650.021.6419735
ACAGCCG13050.021.5121888
Produced by FastQC (version 0.11.5)