FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942926
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA55290.5863662684028227No Hit
ATATATTACTGTGCGAAAGATGAGACTGGCTTCACAATGCCCCGCTGGTT44740.4744805000604501No Hit
ATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG23570.24996659334878876No Hit
GCCATATATTACTGTGCGAAAGATGAGACTGGCTTCACAATGCCCCGCTG22260.23607366855935671No Hit
CTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAATCTGGGGGAGCC19920.21125729908815752No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19690.20881808328543278No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17320.18368355523126947No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16370.17360853343740654No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16200.17180563480061No Hit
ATATTACTGTGCGAAAGATGAGACTGGCTTCACAATGCCCCGCTGGTTCG15760.16713930891713666No Hit
CTACAGAGACTTCGTGAAGGGCCGGTTCACCGTCTCCAGAGACAATTCCA15650.16597272744626831No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15570.16512430455836408No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGCAGCTATGCCATGAGCTGGG15110.16024587295291465No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14690.15579165279141738No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14240.15101927404695598No Hit
TATAAGAACTGGCCTCCGTGGACGTTCGGTCAAGGGACCAAGGTGGAAAT14090.1494284811321355No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCA14080.14932242827114747No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA14040.14889821682719534No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13860.14698926532941078No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT13850.14688321246842276No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13790.14624689530249457No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13750.14582268385854247No Hit
GGACTGAACAGAGAGAACTCCCCATGGAGTTTGGGCTGAGCTGGCTTTTT13390.14200478086297336No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC13230.14030793508716483No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13110.13903530075530846No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13010.13797477214542817No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12850.1362779263696197No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12340.1308692304592301No Hit
GTGTGAGGTGCAGCTGTTGGAATCTGGGGGAGCCTTGGTACAGCCTGGGG12260.13002080757132586No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11980.127051327463661No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA11960.1268392217416849No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11660.12365763591204401No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11530.12227894871919959No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCAGCAGCTATGCC11390.12079420866536716No Hit
GTGCTAGTGCATTCTACAGAGACTTCGTGAAGGGCCGGTTCACCGTCTCC11310.11994578577746293No Hit
GTTCTTATATTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC10950.11612788278189382No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCTTCTCCT10800.11453708986707335No Hit
GTCTCTGGATTCACCTTCAGCAGCTATGCCATGAGCTGGGTCCGCCAGGC10730.11379471984015713No Hit
GATCAGGACTGAACAGAGAGAACTCCCCATGGAGTTTGGGCTGAGCTGGC10660.1130523498132409No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC10620.11262813836928878No Hit
GAACAGAGAGAACTCCCCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10540.11177971548138453No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10410.11040102828854013No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA10160.10774970676383938No Hit
CACTAGCACCATTACCACTAATACCTGAGACCCACTCCAGCCCCTTCCCT9960.10562864954407876No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9930.10531049096111467No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9850.10446206807321042No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT9830.10424996235123435No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9520.10096232366060538No Hit
GTCCCAGACCCACTGCCACTGAACCTGGGTGGGATACCAGAGGCCCTGGT9510.10085627079961736No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6600.072.170831
GTATCAA17400.068.852631
TCAACGC19150.062.0287674
ATCAACG19300.061.9196853
CAACGCA19800.059.628885
TATCAAC20100.059.1503752
AACGCAG21050.056.4299666
GAATACG2550.053.6403279
ACGCAGA23450.050.6546177
CGCAGAG23650.050.226258
GCAGAGT26850.044.5083779
GTAATAT22600.037.3627741
TAATATA23600.036.63862
ATGGCCG22550.036.3945478
TGGCCGT22800.035.995489
TACACCG1201.0036527E-535.9954765
GTACATG22500.035.604281
ATATGGC23650.035.3105746
TATGGCC23450.035.3047337
TACATGG22950.034.8505742
Produced by FastQC (version 0.11.5)