FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942926
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA47500.5037510896931466No Hit
ATATATTACTGTGCGAAAGATGAGACTGGCTTCACAATGCCCCGCTGGTT41470.43980121451736404No Hit
GCCATATATTACTGTGCGAAAGATGAGACTGGCTTCACAATGCCCCGCTG21190.2247260124336374No Hit
ATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG20040.21252993342001386No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17200.1824109208994131No Hit
CTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAATCTGGGGGAGCC17000.1802898636796525No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14720.15610981137438143No Hit
ATATTACTGTGCGAAAGATGAGACTGGCTTCACAATGCCCCGCTGGTTCG13460.14274715088988954No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13300.14105030511408106No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGCAGCTATGCCATGAGCTGGG13040.13829293072839227No Hit
CTACAGAGACTTCGTGAAGGGCCGGTTCACCGTCTCCAGAGACAATTCCA13040.13829293072839227No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT12230.12970264898836176No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12100.12832396179551736No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12050.1277936974905772No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11890.12609685171476873No Hit
GGACTGAACAGAGAGAACTCCCCATGGAGTTTGGGCTGAGCTGGCTTTTT11870.12588474599279265No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11850.12567264027081657No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA11810.12524842882686446No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11690.12397579449500808No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11680.12386974163402006No Hit
GTGTGAGGTGCAGCTGTTGGAATCTGGGGGAGCCTTGGTACAGCCTGGGG11610.12312737160710384No Hit
TATAAGAACTGGCCTCCGTGGACGTTCGGTCAAGGGACCAAGGTGGAAAT11610.12312737160710384No Hit
GTTCTTATATTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC11420.12111236724833127No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCA11370.1205821029433911No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10970.11633998850386987No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10700.11347656125719303No Hit
GTGCTAGTGCATTCTACAGAGACTTCGTGAAGGGCCGGTTCACCGTCTCC10220.10838602392976755No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10170.10785575962482741No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10060.10668917815395906No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10020.10626496671000693No Hit
GTCTCTGGATTCACCTTCAGCAGCTATGCCATGAGCTGGGTCCGCCAGGC10000.10605286098803088No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9810.1040378566292583No Hit
GATCAGGACTGAACAGAGAGAACTCCCCATGGAGTTTGGGCTGAGCTGGC9730.10318943374135404No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9700.10287127515838995No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG9610.10191679940949767No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC9610.10191679940949767No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9470.10043205935566524No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCAGCAGCTATGCC9450.10021995363368918No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG11500.056.961913
TCAACGC12200.053.6936044
CAACGCA12550.053.3433465
GTATCAA13300.052.5643271
GGTATCA5850.050.5125161
AACGCAG14150.048.837776
TATACCG450.00991435647.989895
TATCAAC14700.045.5414242
ACGCAGA16150.042.7897457
CGCAGAG16250.042.083448
GCGTATG700.001087784741.184421
TACGGTC700.001094335341.134195
GTAATAT22850.040.373351
TAATATA24450.038.2741472
ATATGGC25100.036.4226076
ATGGCCG24550.036.3589368
TTACGGT800.002109096935.9924164
GTACATG22250.035.955391
TGGCCGT24750.035.774289
GCAGAGT19150.035.710499
Produced by FastQC (version 0.11.5)