FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117824
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACAGCGGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA56090.5017784552845529No Hit
GTATATTATTGTGCAAGAGATTTTAGGGGATCCAACTACTGGGGCCCGGG32140.2875229016374671No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30840.27589316386121604No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30730.2749091091263025No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27630.2471766575060117No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21400.19144337570136263No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTG19650.1757879594641017No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG18450.16505281690140844No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG18220.16299524791022557No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18120.16210065269666782No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG16950.15163388869804192No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16730.1496657792282148No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC15990.14304577464788734No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15600.13955685331501202No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15370.13749928432382916No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15300.1368730676743387No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA15100.13508387724722318No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14460.12935846788045344No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT14230.12730089888927057No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13840.1238119775563953No Hit
GATTATTACTGCAGCTCATCTACAAGCAGGAACACTTGGGTGTTCGGCGG13730.12282792282148174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.12166494904385662No Hit
GATTAAGACTGATGGGAGTGTGACAACCTACGCGGACTCCGTGAAGGGCC13120.11737089201877934No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT13090.11710251345471202No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT13020.11647629680522158No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCT12960.11593953967708691No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTG12230.10940899461811521No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12160.10878277796862476No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11900.10645683041337456No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11760.1052043971143937No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11570.10350466620863391No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11370.10171547578151838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG27800.057.4909023
TCAACGC28200.056.6754264
CAACGCA28800.055.2447175
AACGCAG29550.054.0861936
ACGCAGA33100.048.0679057
CGCAGAG34150.046.589988
AATATAC21150.044.5914763
ACAGCGG20400.044.466338
CAGCGGT20800.043.611219
GCAGAGT38450.041.3796549
TAATATA23200.041.3033182
TATACAG23600.041.1824955
ATAATAT23200.040.7405781
ATATACA24400.039.2421464
CCCCGTT7350.039.1799247
CCCGTTG7500.037.436428
ACCCCGT7700.037.399026
CCGTTGA7750.036.2287949
GTATCAA47600.034.8630031
TATCAAC49500.032.8945962
Produced by FastQC (version 0.11.5)