FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195775
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGTCTACTCAGCTCCATGTAGG43810.36637327256381846No Hit
GTAATAATCAGCCTCATCCTCAGACCGGAGCCCACTGATGGCCAGGGAGG41210.3446300516401497No Hit
GATTATTACTGTGCATCTTGGGATGACAGCCTCAATGGTCATGTCTTCGG34920.292028182559428No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC30770.25732265685434136No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA30570.2556501013986745No Hit
CTACTACGCTATGGACGTTTGGGGCCAAGGGACCCCGGTCACCGTCTCCA27850.23290334720160563No Hit
GTGTATTACTGTGCGAGATGGGGGGCCGTGGTACAAGAGGAACGACCGTC27570.23056176956367208No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27440.22947460851748866No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25400.212414542869687No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23840.19936861031548575No Hit
GCCTACATGGAGCTGAGTAGACTGAGATCTGACGACACGGCCGTGTATTA22800.19067132194601827No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21960.1836465890322176No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21390.17887980598356715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20970.1753674395266668No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20760.17361125629821664No Hit
CTCTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA20740.17344400075264996No Hit
GCGTAGTAGTAGTAGTCAGACGGTCGTTCCTCTTGTACCACGGCCCCCCA20540.17177144529698313No Hit
CTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGG20450.17101879534193307No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20430.1708515397963664No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC20400.17060065647801637No Hit
GTACAAGAGGAACGACCGTCTGACTACTACTACTACGCTATGGACGTTTG19980.16708829002111603No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG19690.1646630846103991No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19140.16006355710731535No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT19090.15964541824339862No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG18700.15638393510484833No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA18660.15604942401371494No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18460.15437686855804814No Hit
GTATTACTGTGCGAGATGGGGGGCCGTGGTACAAGAGGAACGACCGTCTG18380.1537078463757814No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17570.14693399678033073No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17510.14643223014363071No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT17450.14593046350693065No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTGAGG16850.14091279713993018No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16770.14024377495766344No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT16450.13756768622859653No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATGACCATTGAGGC16100.13464071418117957No Hit
GAGTAGACTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGATGGG16080.13447345863561289No Hit
CCCTATGTCTGGAACAACAAATTACTCACAGAAGTTCCAGGGCAGAGTCA15990.1337208086805628No Hit
CCTCATACACTCTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCT15720.13146285881541261No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15470.12937216449582906No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15430.1290376534046957No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCC15130.12652882022119546No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15090.12619430913006208No Hit
ACATGGAGCTGAGTAGACTGAGATCTGACGACACGGCCGTGTATTACTGT14780.1236018481737785No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG14780.1236018481737785No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14730.12318370930986179No Hit
CTACTACTACGCTATGGACGTTTGGGGCCAAGGGACCCCGGTCACCGTCT14590.12201292049089502No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC14270.1193368317618281No Hit
GACTACTACTACTACGCTATGGACGTTTGGGGCCAAGGGACCCCGGTCAC14260.11925320398904475No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14240.11908594844347807No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG14000.11707888189667788No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13980.1169116263511112No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13980.1169116263511112No Hit
ATACACGGCCGTGTCGTCAGATCTCAGTCTACTCAGCTCCATGTAGGCCG13880.11607534862327779No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGTCTACTCAGCTCCATGTA13880.11607534862327779No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13840.11574083753214442No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13830.11565720975936107No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT13690.1144864209403943No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCCTCCCTATGTCTGGA13640.11406828207647761No Hit
TTATTACTGTGCATCTTGGGATGACAGCCTCAATGGTCATGTCTTCGGAA13540.1132320043486442No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13330.11147582112019402No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG12700.1062072714348435No Hit
GCCGTGTATTACTGTGCGAGATGGGGGGCCGTGGTACAAGAGGAACGACC12690.10612364366206017No Hit
AGCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGG12580.1052037381614434No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12510.10461834375196002No Hit
TAGTAGTAGTAGTCAGACGGTCGTTCCTCTTGTACCACGGCCCCCCATCT12500.10453471597917668No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12410.1037820660241266No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12360.10336392716020991No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC12270.10261127720515982No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG12060.10085509397670966No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12030.10060421065835964No Hit
GCTACAGGTGTCCAGTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGT12020.1005205828855763No Hit
GTAGTAGTCAGACGGTCGTTCCTCTTGTACCACGGCCCCCCATCTCGCAC12010.10043695511279295No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11980.10018607179444294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.077.6178051
GGTATCA8000.072.119871
ATCAACG27050.063.1115233
TCAACGC27300.062.2697224
CAACGCA27750.060.9978145
TATCAAC28650.060.0898132
AACGCAG28550.059.2414936
CGCAGAG32200.052.526238
CAGCGCT6550.047.2488679
ACGCAGA39200.043.3301477
GCAGCGC7750.039.004248
AGCGCTG6050.034.5264555
GGCAGCG8550.034.5129437
GTACATG31750.031.800891
CAGAGTA35550.031.1777949
ACATGGG31800.030.3532523
GAATACG2202.431807E-829.4431369
GCAGAGT61050.028.2937059
GTATGTA16750.027.9868181
GTACACG15500.027.4176185
Produced by FastQC (version 0.11.5)