FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195775
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGTCTACTCAGCTCCATGTAGG45990.38460412703058683No Hit
GATTATTACTGTGCATCTTGGGATGACAGCCTCAATGGTCATGTCTTCGG32730.27371370031987624No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC27750.23206706947377226No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27720.2318161861554222No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA26670.22303527001317136No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26330.2201919257385378No Hit
GTGTATTACTGTGCGAGATGGGGGGCCGTGGTACAAGAGGAACGACCGTC25550.21366895946143713No Hit
CTACTACGCTATGGACGTTTGGGGCCAAGGGACCCCGGTCACCGTCTCCA22950.1919257385377684No Hit
CTCTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA22870.19125671635550165No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22380.18715895548911793No Hit
CTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGG22100.18481737785118438No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21990.18389747235056764No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT21660.18113775584871736No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT20600.17227321193368317No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20560.1719387008425498No Hit
GCCTACATGGAGCTGAGTAGACTGAGATCTGACGACACGGCCGTGTATTA20400.17060065647801637No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCTGGGACCC20040.16759005665781607No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19510.16315778470029896No Hit
GCGTAGTAGTAGTAGTCAGACGGTCGTTCCTCTTGTACCACGGCCCCCCA19070.15947816269783194No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18320.15320607973908135No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17760.14852292446321422No Hit
GTACAAGAGGAACGACCGTCTGACTACTACTACTACGCTATGGACGTTTG17690.14793753005373084No Hit
CCTCATACACTCTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCT17650.14760301896259748No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17440.1458468357341473No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17110.14308711923229706No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16940.14166544709498025No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16610.13890573059312997No Hit
GTATTACTGTGCGAGATGGGGGGCCGTGGTACAAGAGGAACGACCGTCTG16520.1381530806380799No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16380.13698229181911314No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC16160.1351424808178796No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA16150.13505885304509627No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16030.13405531977169616No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG15390.12870314231356234No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14900.1246053814471786No Hit
CCCTATGTCTGGAACAACAAATTACTCACAGAAGTTCCAGGGCAGAGTCA14540.12159478162697832No Hit
ATACACGGCCGTGTCGTCAGATCTCAGTCTACTCAGCTCCATGTAGGCCG14490.12117664276306161No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14470.12100938721749492No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC14060.11758064853337793No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14020.11724613744224456No Hit
GAGTAGACTGAGATCTGACGACACGGCCGTGTATTACTGTGCGAGATGGG13780.11523907089544438No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13780.11523907089544438No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13690.1144864209403943No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13600.11373377098534423No Hit
GTGCTGACTCAGCCACCCTCAACGTCTGGGACCCCCGGGCAGAGGGTCAC13590.1136501432125609No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC13400.11206121552967742No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13140.10988689343731053No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT13020.10888336016391044No Hit
GACTACTACTACTACGCTATGGACGTTTGGGGCCAAGGGACCCCGGTCAC12900.10787982689051034No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTCAGTCTACTCAGCTCCATGTA12830.10729443248102695No Hit
CATGTAGGCCGTGCTCGTGGATGCGTCCGCGGTAATGCTGACTCTGCCCT12660.10587276034371015No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT12490.10445108820639334No Hit
TCATTAGAGTGTATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGGCGGTA12490.10445108820639334No Hit
CCATGTAGGCCGTGCTCGTGGATGCGTCCGCGGTAATGCTGACTCTGCCC12470.10428383266082666No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12410.1037820660241266No Hit
TTATTACTGTGCATCTTGGGATGACAGCCTCAATGGTCATGTCTTCGGAA12380.10353118270577659No Hit
ACATGGAGCTGAGTAGACTGAGATCTGACGACACGGCCGTGTATTACTGT12360.10336392716020991No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC12330.10311304384185989No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12310.10294578829629321No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCCTCCCTATGTCTGGA12230.10227676611402646No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG12230.10227676611402646No Hit
ACTCAGCTCCATGTAGGCCGTGCTCGTGGATGCGTCCGCGGTAATGCTGA12220.10219313834124313No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12200.10202588279567644No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12120.10135686061340972No Hit
CTACTACTACGCTATGGACGTTTGGGGCCAAGGGACCCCGGTCACCGTCT11970.10010244402165959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)