FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610089
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA14820.24291537792027065No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13980.22914689496122695No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9590.15719018044908203No Hit
ATTAGAAGGTGTCCAGTGTGACGTGCAGCTGGTGGACTCTGGGGGAGGCT8970.14702772874121645No Hit
ATCCCATACTCCGCAGTAATAATCGGCCTCGTCCCCGGTCTGGAGTCCGC7920.12981712504241183No Hit
GTATGGGATAGCAGCCTGAATGTTTGCGTTTTTGGCGGGGGGACCAAGCT7410.12145768896013533No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT6340.10391926423849636No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6290.10309971168141042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC1355.251968E-737.3263
GTGCTAG1459.1122456E-734.7888641
GTATCAA11500.031.9582181
ATCAACG11550.031.1627123
TCAACGC11550.031.1627124
CAACGCA11850.030.373785
AACGCAG12500.029.9461216
TATCAAC12800.028.6818662
CGCAGAG13750.027.7472788
GTATGGG5450.026.445011
TACTCCG5450.026.4168327
CTATACT2207.9570964E-726.1766764
GTATATA3451.1641532E-1025.0652681
TATACTG3451.1823431E-1025.038565
GCAGAGT15800.024.1471569
GTACATG18250.023.6918281
GTAGTAC1851.906105E-423.3716681
ATACTCC7400.023.3467676
ACTCCGC5900.023.1818878
TACATGG18050.023.1311912
Produced by FastQC (version 0.11.5)