FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776203
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCGCCGCTCAGGCTGTCCATTTGCAGGTGGA51360.6616825753056867No Hit
GTATATTACTGTGCGAAATTTGCCCGAGCGTGTAGTGGTGATAACTGCTA37210.4793849031761021No Hit
ACCATGGACTGTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGG34510.44460018835278914No Hit
GTATGTGCTACTTCCACCTCTATAAATAACTGAGACCCACTCCAGCCCCT23870.3075226454935114No Hit
AGCACATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA22350.2879401393707574No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21550.2776335572008869No Hit
ATATACGGCCGTGTCGTCGCCGCTCAGGCTGTCCATTTGCAGGTGGAGTG21510.27711822809239334No Hit
GCCGTATATTACTGTGCGAAATTTGCCCGAGCGTGTAGTGGTGATAACTG20190.260112367512107No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19910.25650506375265236No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC19080.2458119847514117No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18360.23653606079852824No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17310.22300867170057317No Hit
ATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATT16730.21553639962741702No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16650.21450574141043No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16210.20883712121700124No Hit
GCATAGTATGTGCTACTTCCACCTCTATAAATAACTGAGACCCACTCCAG16100.207419966168644No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16100.207419966168644No Hit
GTATAGTAGCTCACCTTTCGGTTTCGGCCCTGGGACCAAAGTGGATATCA15310.1972422162758969No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15250.19646922261315664No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15160.19530973211904618No Hit
CTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCCA13630.17559839371916883No Hit
GTCTGCATAGTATGTGCTACTTCCACCTCTATAAATAACTGAGACCCACT12150.15653121670490838No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11720.150991428788603No Hit
GGACTGAACAGAGAGGACTTACCATGGACTGTGGGCTGAGCTGGCTTTTT11680.15047609968010944No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT11550.14880128007750548No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11520.14841478324613536No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11450.14751295730627167No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11410.14699762819777817No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT11310.14570930542654434No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11310.14570930542654434No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11070.1426173307755832No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10810.13926769157037527No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10680.1375928719677713No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10350.13334140682269974No Hit
CCCTGAGACTCTCCTGTGCAACCTCTGGATTCGTCTTTAGCAACTACGTC10040.1293476062318749No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT10030.1292187739547515No Hit
CTCCTGTGCAACCTCTGGATTCGTCTTTAGCAACTACGTCATGAGCTGGG9960.12831694801488785No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG9880.1272862897979008No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9750.12561147019529684No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCT9680.12470964425543318No Hit
ATATTACTGTGCGAAATTTGCCCGAGCGTGTAGTGGTGATAACTGCTACT9670.1245808119783098No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9610.12380781831556951No Hit
GATCAGGACTGAACAGAGAGGACTTACCATGGACTGTGGGCTGAGCTGGC9500.1223906632672123No Hit
CTACACGCTCGGGCAAATTTCGCACAGTAATATACGGCCGTGTCGTCGCC9470.12200416643584217No Hit
CCACTACACGCTCGGGCAAATTTCGCACAGTAATATACGGCCGTGTCGTC9370.12071584366460834No Hit
GAACAGAGAGGACTTACCATGGACTGTGGGCTGAGCTGGCTTTTTCTTGT9200.11852569495351087No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8960.11543372030254971No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8910.11478955891693281No Hit
ATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCCAAG8860.11414539753131589No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8830.11375890069994576No Hit
CTACTATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCT8810.11350123614569899No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC8680.11182641654309505No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8560.11028042921761445No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8470.10912093872350404No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8430.10860560961501049No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC8390.10809028050651698No Hit
CTATTACTGTCAGCAGTATAGTAGCTCACCTTTCGGTTTCGGCCCTGGGA8390.10809028050651698No Hit
CAGTAATATACGGCCGTGTCGTCGCCGCTCAGGCTGTCCATTTGCAGGTG8390.10809028050651698No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8340.10744611912090007No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGACGTAGTTGC8250.10628662862678963No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG8200.10564246724117272No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7870.10139100209610113No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7860.10126216981897777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.1003603438791141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTC300.001990757471.988087
ACGGTCG300.001990757471.988088
TCAACGC17800.056.226284
ATCAACG17950.055.756423
TAACCGT400.00622237453.9910627
CAACGCA18750.053.377485
TATCAAC19350.051.7223662
GGTAACG701.7682372E-551.420067
AACGCAG19450.051.086256
GTAACGA605.1202066E-447.9920588
CGCAGAG21100.047.4234288
GCAGAGT22900.043.3814669
GTATCAA25100.042.7697751
CAGCGCT5850.041.8392269
TAACGAC700.001093972741.1360479
GCAGCGC6550.039.5659688
TATACGG21400.036.6739125
TACGGCC21600.035.3274847
ATACGGC21650.035.252716
GGCAGCG7200.034.994217
Produced by FastQC (version 0.11.5)