FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776203
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCGTCGCCGCTCAGGCTGTCCATTTGCAGGTGGA51780.6670935309448688No Hit
GTATATTACTGTGCGAAATTTGCCCGAGCGTGTAGTGGTGATAACTGCTA37220.47951373545322545No Hit
ACCATGGACTGTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGG33760.4349377675685355No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25480.32826464211037576No Hit
GTATGTGCTACTTCCACCTCTATAAATAACTGAGACCCACTCCAGCCCCT24420.3146084207352973No Hit
AGCACATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA21890.28201385462308187No Hit
ATATACGGCCGTGTCGTCGCCGCTCAGGCTGTCCATTTGCAGGTGGAGTG20760.2674558073081397No Hit
GCCGTATATTACTGTGCGAAATTTGCCCGAGCGTGTAGTGGTGATAACTG20250.2608853611748473No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19790.25495907642717175No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19050.24542548792004154No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18490.2382108804011322No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16960.21849954200125482No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16490.21244442497645588No Hit
GCATAGTATGTGCTACTTCCACCTCTATAAATAACTGAGACCCACTCCAG15940.20535864973466994No Hit
ATACTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATT15900.20484332062617638No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15510.19981886181836456No Hit
GTATAGTAGCTCACCTTTCGGTTTCGGCCCTGGGACCAAAGTGGATATCA14840.19118709925109797No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14830.1910582669739746No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14090.18152467846684436No Hit
CTATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCCA13260.17083159946560372No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13100.1687702830316296No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13080.16851261847738286No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12140.156402384427785No Hit
GGACTGAACAGAGAGGACTTACCATGGACTGTGGGCTGAGCTGGCTTTTT12080.1556293907650447No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT11910.15343924205394724No Hit
GTCTGCATAGTATGTGCTACTTCCACCTCTATAAATAACTGAGACCCACT11870.1529239129454537No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11790.15189325472846665No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11780.15176442245134328No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11650.15008960284873932No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11200.14429215037818716No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10950.1410713434501026No Hit
CCCTGAGACTCTCCTGTGCAACCTCTGGATTCGTCTTTAGCAACTACGTC10360.1334702390998231No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10310.1328260777142062No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10120.13037826444886197No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9910.12767278662927095No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9900.12754395435214755No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9790.1261267993037904No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9760.12574030247242024No Hit
ATATTACTGTGCGAAATTTGCCCGAGCGTGTAGTGGTGATAACTGCTACT9760.12574030247242024No Hit
CTACACGCTCGGGCAAATTTCGCACAGTAATATACGGCCGTGTCGTCGCC9740.12548263791817346No Hit
CTCCTGTGCAACCTCTGGATTCGTCTTTAGCAACTACGTCATGAGCTGGG9540.12290599237570585No Hit
GATCAGGACTGAACAGAGAGGACTTACCATGGACTGTGGGCTGAGCTGGC9510.1225194955443357No Hit
CTACTATACTGCTGACAGTAATAGACTGCAAAATCTTCAGGCTCCAGTCT9500.1223906632672123No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9120.11749503673652381No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACGCTCTCCT9110.11736620445940045No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG9100.11723737218227706No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9090.11710853990515367No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG9050.11659321079666016No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8920.1149183911940562No Hit
CCACTACACGCTCGGGCAAATTTCGCACAGTAATATACGGCCGTGTCGTC8820.11363006842282238No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8800.11337240386857562No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8660.11156875198884827No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8530.10989393238624431No Hit
GAACAGAGAGGACTTACCATGGACTGTGGGCTGAGCTGGCTTTTTCTTGT8480.10924977100062741No Hit
ATGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGATAATTCCAAG8250.10628662862678963No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8180.10538480268692596No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGACGTAGTTGC8080.10409647991569215No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC8050.103709983084322No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8040.10358115080719864No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7900.10177749892747129No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7790.1003603438791141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG450.009895666548.0120125
ATCAACG13200.046.375243
TCAACGC13250.045.6567044
TATCAAC15050.042.5914732
CAACGCA14700.040.6632355
TATACGG20100.040.4877435
GTAATAT21050.040.3789561
ATACGGC20000.040.3170936
AACGCAG15000.039.8371286
CGCAGAG15050.039.7073368
GGCAGCG4200.039.4257287
TACGGCC20500.039.333757
AATATAC21300.038.882973
GTACACG10400.037.3939745
TAATATA23050.036.8706052
TACACGG10650.036.5044146
ATATACG23100.036.164894
GTGCTAG1604.8565198E-836.0159721
ACACGGG11050.035.8345227
GTATGTA11300.035.697251
Produced by FastQC (version 0.11.5)