FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841293
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAAGGCGCTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA36160.42981458302874265No Hit
TTGTATTACTGTGTAAAAGATGGGGCAACAAATGTAGCACAAGGAAGCAA26320.3128517650806556No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24620.29264477417499013No Hit
GTATGTGCTACCACCATCCCAACTAATAAGAGAGACCCACTCCAGACCCT18210.2164525319953928No Hit
AGCACATACTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGA18020.21419410360005373No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTTGTGCTG17390.20670563049971888No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGCGTGGCTGTGTTCCCAGAGTT15940.1894702559037101No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA15440.18352702328439677No Hit
CCCCTGTGCTGGTCATCTATCAAGACAGCCAGCGGCCCTCAGGGATCCCT15090.1793667604508774No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT15030.1786535725365598No Hit
TGATTATACCATGTACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGT14850.17651400879360699No Hit
ACCATGTACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGTGGGTCTC14730.1750876329649718No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14390.17104623478383868No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14130.16795575382179573No Hit
CAGTAATACAAGGCGCTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA13810.16415208494543518No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13790.16391435564066265No Hit
GTCTGAGAACTGAGGACAGCGCCTTGTATTACTGTGTAAAAGATGGGGCA13380.15904090489282569No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA13200.15690134114987286No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12950.1539297248402162No Hit
GTGGTAGCACATACTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCC12450.14798649222090282No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12430.1477487629161303No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12200.14501487591124612No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12180.14477714660647362No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12100.14382622938738346No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATACTATGCAGACTCTGTGAGG11920.14168666564443066No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11890.14133007168727185No Hit
GTATTACTGTGTAAAAGATGGGGCAACAAATGTAGCACAAGGAAGCAACT11770.13990369585863666No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11660.1385961846823877No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT11640.13835845537761515No Hit
GTACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGTGGGTCTCTCTTA11610.13800186142045637No Hit
TTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG11300.1343170571964821No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11290.13419819254409582No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10950.13015679436296274No Hit
ATACTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACA10880.12932474179625886No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10710.12730404270569232No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10650.1265908547913747No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10420.12385696778649057No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTTGTGCTGC10400.12361923848171802No Hit
GTAATAGTCAGCCTCATCCATAGCCTGGGTCCCGCTGATGGTCAGCGTGG10210.12136081008637895No Hit
CTTTTACACAGTAATACAAGGCGCTGTCCTCAGTTCTCAGACTGTTCATT10140.12052875751967507No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACAA10100.12005329891013No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9890.11755714121001838No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9880.11743827655763212No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9780.11624963003376944No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9760.11601190072899692No Hit
GCATAGTATGTGCTACCACCATCCCAACTAATAAGAGAGACCCACTCCAG9720.11553644211945184No Hit
GAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTCCTTGT9700.11529871281467932No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9560.11363460768127155No Hit
GTGTAAAAGATGGGGCAACAAATGTAGCACAAGGAAGCAACTACGGTATG9430.11208936720025009No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9380.11149504393831876No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTGG9360.11125731463354621No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9360.11125731463354621No Hit
GATCAGCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGG9320.11078185602400115No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9280.11030639741445608No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9270.11018753276206981No Hit
GTGCTACCACCATCCCAACTAATAAGAGAGACCCACTCCAGACCCTTCCC9190.10923661554297968No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9100.10816683367150326No Hit
GACTATTACTGTCAGGCGTGGGACAGCAGCACAAATTGGGTGTTCGGCGG9090.108047969019117No Hit
GGTCTGGAGTGGGTCTCTCTTATTAGTTGGGATGGTGGTAGCACATACTA9090.108047969019117No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9060.1076913750619582No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9050.10757251040957193No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9040.10745364575718566No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8950.10638386388570925No Hit
GTGCTGGTCATCTATCAAGACAGCCAGCGGCCCTCAGGGATCCCTGAGCG8940.10626499923332298No Hit
GCACTGAACACAGAGAACGCACCATGGAGTTTGGACTGAGCTGGGTTTTC8910.10590840527616419No Hit
CTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACAGCA8910.10590840527616419No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTG8810.10471975875230152No Hit
GTATAATCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA8800.10460089409991524No Hit
CTCCAGACCCTTCCCCGGAGTTTGACGGACCCAGTACATGGTATAATCAT8790.10448202944752898No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8760.10412543549037018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.10269905966173497No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8510.1011538191807135No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8500.10103495452832723No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8470.10067836057116841No Hit
ATCTTTTACACAGTAATACAAGGCGCTGTCCTCAGTTCTCAGACTGTTCA8450.1004406312663959No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)