FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences841293
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACAAGGCGCTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA29820.35445439341584917No Hit
TTGTATTACTGTGTAAAAGATGGGGCAACAAATGTAGCACAAGGAAGCAA22000.26150223524978816No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21800.2591249422020628No Hit
GTATGTGCTACCACCATCCCAACTAATAAGAGAGACCCACTCCAGACCCT15830.18816274472746117No Hit
AGCACATACTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGA15730.1869740982035985No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTTGTGCTG15130.17984221906042247No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT13090.15559382997362392No Hit
CCCCTGTGCTGGTCATCTATCAAGACAGCCAGCGGCCCTCAGGGATCCCT12940.1538108601878299No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA12790.1520278904020359No Hit
TCCATAGCCTGGGTCCCGCTGATGGTCAGCGTGGCTGTGTTCCCAGAGTT12700.15095810853055952No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12690.15083924387817324No Hit
ACCATGTACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGTGGGTCTC12570.14941286804953802No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12470.14822422152567535No Hit
CCTATGAGCTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA12100.14382622938738346No Hit
TGATTATACCATGTACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGT12030.1429941768206796No Hit
CTCTTATTAGTTGGGATGGTGGTAGCACATACTATGCAGACTCTGTGAGG12020.14287531216829333No Hit
GTCTGAGAACTGAGGACAGCGCCTTGTATTACTGTGTAAAAGATGGGGCA11850.1408546130777268No Hit
CAGTAATACAAGGCGCTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA11520.13693207954897998No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11480.1364566209394349No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10580.12575880222467084No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10570.12563993757228456No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10260.12195513334831028No Hit
GTATTACTGTGTAAAAGATGGGGCAACAAATGTAGCACAAGGAAGCAACT10240.12171740404353774No Hit
GTGGTAGCACATACTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCC10210.12136081008637895No Hit
GTACTGGGTCCGTCAAACTCCGGGGAAGGGTCTGGAGTGGGTCTCTCTTA10010.1189835170386536No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9960.11838919377672227No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9470.11256482580979515No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCCTGAGATTCCCAGGTGT9410.11185163789547756No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9320.11078185602400115No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9120.1084045629762758No Hit
ATACTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACA8840.10507635270946031No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTTGTGCTGC8750.10400657083798391No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGGCGTGGGACAGCAGCACAA8730.10376884153321136No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8720.10364997688082511No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8650.10281792431412123No Hit
GTATAATCATCAAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA8580.10198587174741736No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8550.10162927779025857No Hit
GGTCTGGAGTGGGTCTCTCTTATTAGTTGGGATGGTGGTAGCACATACTA8490.10091608987594096No Hit
CTTTTACACAGTAATACAAGGCGCTGTCCTCAGTTCTCAGACTGTTCATT8490.10091608987594096No Hit
GTGTAAAAGATGGGGCAACAAATGTAGCACAAGGAAGCAACTACGGTATG8460.10055949591878217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9450.051.801143
TCAACGC9650.049.2355544
CAACGCA10650.043.936555
AACGCAG11200.043.7072146
ACGCAGA11500.042.5670247
CGTTATT700.001094005141.13622
CGCAGAG11800.040.874748
GAATACG2002.419256E-1035.9941759
GCAGAGT14400.034.494429
TATTGAG9950.034.0045435
ATGGGAG8800.031.9039295
TTATTGA10850.031.1838934
ATTAGAC1804.9649407E-627.9954723
GGTTTAT8500.027.9683671
AGTACCG1050.00797877727.4241335
ACATGGG22700.027.2731193
AAGGCGC21500.026.1167038
AGGCGCT21800.026.0875239
ATTGAGT8100.025.7736076
GTATAAC1400.001037158925.7284761
Produced by FastQC (version 0.11.5)