FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369323
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGACAATGGAATAACTACTTTGACTACTGGGGCCA110890.46802398828694947No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGATCTCACCTTCAGGGAGA99930.42176604878271134No Hit
GTCTCTGGTGACTCCATCAGCAGTAGCAATTACTACTGGGGCTGGATCCG73540.31038402108956864No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT58630.2474546526581644No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49760.21001779833311035No Hit
GCTTAATAATTACCTCTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA49250.2078652847247927No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47750.20153436234738786No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC45370.1914892988419055No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT43810.1849051395694044No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC43280.18266821366272137No Hit
ATTACAACCCGTCGCTCAAGAGTCGAGTCATCATATCCATAGACACGTCC43110.18195070912661548No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA40190.16962651356526737No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA39140.16519486790108398No Hit
TCCATAGACACGTCCACGAACCAGTTCTCCCTGAAGGTGAGATCTGTGAC38350.16186058211565074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.15789320409247704No Hit
TTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT36520.15413685681521683No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC36450.15384141377093793No Hit
ATTTTAGTGAGAGCACCTATTACAACCCGTCGCTCAAGAGTCGAGTCATC35750.150886983328149No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG35700.1506759525822355No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG32910.1389004369602625No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT32260.13615703726338704No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31760.13404672980425208No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG31130.13138774240574205No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30720.1296572902892514No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAA30190.12742036438256835No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29690.1253100569234334No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG29680.1252678507742507No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTAGCAATTACTACTGGG29520.12459255238732753No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT27620.1165733840426147No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27210.11484293192612403No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA26800.11311247980963339No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26470.11171967688660431No Hit
ATAATTACCTCTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA25850.10910289563727697No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25780.10880745259299808No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG25390.10716141277487283No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA25260.10661273283549774No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG24900.10509331146492057No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT24790.10462904382391089No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24430.10310962245333373No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC24160.10197005642540084No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA23930.10099931499419877No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23880.10078828424828527No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)