FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2369323
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGACAATGGAATAACTACTTTGACTACTGGGGCCA96700.4081334625966996No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGATCTCACCTTCAGGGAGA94030.3968644207649189No Hit
GTCTCTGGTGACTCCATCAGCAGTAGCAATTACTACTGGGGCTGGATCCG66760.28176825194369864No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT54550.23023454379162317No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47580.20081685781128197No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43460.18342792434800997No Hit
GCTTAATAATTACCTCTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA42560.17962937092156703No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT39890.1683603290897864No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA38700.16333779733704523No Hit
ATTACAACCCGTCGCTCAAGAGTCGAGTCATCATATCCATAGACACGTCC38280.16156513907137185No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC37380.15776658564492896No Hit
TTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT36220.15287067233973586No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35230.14869226357064866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33110.13974455994391646No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC32700.1380141078274258No Hit
ATTTTAGTGAGAGCACCTATTACAACCCGTCGCTCAAGAGTCGAGTCATC32450.13695895409785833No Hit
TCCATAGACACGTCCACGAACCAGTTCTCCCTGAAGGTGAGATCTGTGAC31850.1344265851468964No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31180.13159877315165558No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28650.1209206174084327No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT28560.12054076206578841No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28290.11940119603785554No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28260.11927457759030745No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28210.11906354684439395No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAA27800.11733309472790328No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27180.11471631347857594No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG26810.11315468595881609No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG26750.11290144906371989No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT26570.11214173837843132No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA26190.11053790470948875No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26050.10994701862093097No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTAGCAATTACTACTGGG25410.10724582507323822No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25310.10682376358141124No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT25150.10614846519448803No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24330.10268756096150672No Hit
ATAATTACCTCTTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA24230.10226549946967974No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23730.10015519201054478No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)