FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679651
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACAGCCGTGTCCTCGACTCTCAGAGTGTTCATTTGCAGATACA22530.3314936636597312No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18400.2707271820390171No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17400.2560137482325488No Hit
GTATATTATTGTGCAAGAGATTTAAGGGGATCCAACCACTGGGGCCAGGG14310.21054923777056164No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13880.2042224612337803No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13660.20098550579635724No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG12390.18229944486214247No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11970.17611980266342578No Hit
ATATACAGCCGTGTCCTCGACTCTCAGAGTGTTCATTTGCAGATACAGCG11610.1708229664930972No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCTTG10780.15861081643372849No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10350.1522840398969471No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC9670.14227890490854866No Hit
GCTGTATATTATTGTGCAAGAGATTTAAGGGGATCCAACCACTGGGGCCA9130.13433365065305575No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8540.12565272470723945No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA8090.1190316794943287No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8080.11888454515626401No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7550.1110864252388358No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7530.11079215656270645No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG7350.10814373847754216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7250.10667239509689533No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7080.1041711113497957No Hit
GATTAAGACTGATGGGAGTGTGACAACCTACGCGGACTCCGTGAAGGGCC6850.100787021574308No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG6820.10034561856011395No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12300.065.553174
ATCAACG12600.063.9923823
CAACGCA13000.062.5771685
AACGCAG14250.056.5827376
ACGCAGA15100.053.3976147
CGCAGAG16050.050.2370158
TACCGTA450.00990958647.994297
GCAGAGT19050.042.7035759
TACACCG700.001093626741.137965
CCGTTGA3600.039.9952439
GTATCAA21150.039.9131361
GTATAGG1851.0186341E-1039.000361
CAGAGCG10150.038.3008589
CCCGTTG3850.037.3981488
ACAGAGC12550.035.5654878
TATCAAC22900.035.5581632
ATAGGTG8100.033.7737544
ACCCCGT4300.033.4843866
TAGGTGG9050.032.6149025
CCCCGTT4450.032.3556987
Produced by FastQC (version 0.11.5)