FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673164
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC67590.4039651821339689No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT51150.30570822704767736No Hit
GACCTAGGATGGTGACCTTGGTCCCAGTTCCAAAGACGAGAGCGCTGGTG48190.2880171937718No Hit
GTACCAACACCACCCAGGCAAAGCCCCCAAACTCATAATTTATGATGTCA47150.28180142532351876No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA45030.26913082040971475No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGACGTT38870.23231434575451063No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC38320.2290271605174388No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT38020.22723415038812694No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT37130.22191488700450168No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA36710.21940467282346499No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC35080.2096626511208704No Hit
GATTATTACTGCAGCTCATATAGAAGCACCAGCGCTCTCGTCTTTGGAAC34620.20691336892259216No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG34340.20523989280190105No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30670.18330540221998562No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC30610.1829468001941232No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29800.1781056728449811No Hit
ATCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29540.17655173073291083No Hit
ACCTAGGATGGTGACCTTGGTCCCAGTTCCAAAGACGAGAGCGCTGGTGC29530.17649196372860043No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29480.17619312870704842No Hit
GTCTAACTCGGGGACCCCTGGGTGATGCTTTTGATATCTGGGGCCAAGGG29400.17571499267256527No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC29380.1755954586639445No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT29290.1750575556251509No Hit
GGTTATAACTATGTCGCCTGGTACCAACACCACCCAGGCAAAGCCCCCAA28920.17284617646566625No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT27980.167228078060489No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC27770.16597297096997068No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27330.1633432227803132No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26970.16119161062513895No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG26960.16113184362082858No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT26880.1606537075863454No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26850.16047440657341422No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA26580.1588606974570335No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGGTTATAACTATGTCGCCT26400.15778489137944637No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26260.15694815331910084No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG26120.15611141525875527No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG25180.150493316853578No Hit
GTGTTGGTACCAGGCGACATAGTTATAACCACCAACGTCACCGCTGGTTC23930.14302244131477848No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22740.13591016780184131No Hit
GCCTTGGGCTGACCTAGGATGGTGACCTTGGTCCCAGTTCCAAAGACGAG22400.13387808965528783No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC22300.13328041961218384No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22280.13316088560356307No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT21940.13112880745700958No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC21040.12574977706907392No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA20960.12527164103459076No Hit
TAACTATGTCGCCTGGTACCAACACCACCCAGGCAAAGCCCCCAAACTCA20720.12383723293114123No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20610.12317979588372689No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20570.1229407278664853No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20550.1228211938578645No Hit
GAAATAGACGGCCGTGTCTTCGGCTCTCAGGCTGTCCATTTGCAGAGACA20010.1195937756251031No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT19970.1193547076078615No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19770.11815936752165358No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG19570.11696402743544566No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT19390.11588822135785853No Hit
GAGTTAGACAGGCCAAAACCGCACAGAAATAGACGGCCGTGTCTTCGGCT19360.11570892034492733No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT19110.11421474523716742No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG18650.1114654630388892No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC18330.10955291890095652No Hit
GGACTGAACAGAGAGAACTCACCATGGGGTTTGGGCTGACCTGGCTTTTT18170.10859664683199018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.10680363670267827No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT17640.10542899560353916No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17290.1033371504526753No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA17250.10309808243543371No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17180.10267971340526094No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAACTATGCCATGAGCTGGG16820.10052810125008667No Hit
CCCCTGGGTGATGCTTTTGATATCTGGGGCCAAGGGACACTGCTCACCGT16800.10040856724146588No Hit
GTTATAACCACCAACGTCACCGCTGGTTCCAGTGCAGGAGATGGTGATCG16740.10004996521560348No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)