FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779112
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTACTACTACCCCACTTTGGACGTCTGGGGCCAAGGGACCACGG43340.24360467469164393No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACACGGGCTTGAGTGGATGG42300.23775906182410103No Hit
ACTATATACACTGGGTGCGACAGGCCCCTGGACACGGGCTTGAGTGGATG41810.23500487883843174No Hit
GTAATAAACGGCCGTGTCGTCGGAGGACAGTCTGCTCACCTCCATGTAGG40660.22854097999451414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.20588923013278534No Hit
TAGTAATACTTGGCACTGTAGTCCAAGTTTGAGCCCACATCTCTCGCACA32010.17992121912504666No Hit
GTTTATTACTGTGCGAGAGATGTGGGCTCAAACTTGGACTACAGTGCCAA29570.16620651201273443No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26650.14979382973078706No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26570.1493441672025145No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGAGGGTCTCCTGCA25090.14102541042947267No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC25020.14063195571723422No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23840.13399943342521436No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22000.12365719527494615No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21850.12281407803443516No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21730.12213958424202637No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21700.12197096079392415No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21100.11859849183188018No Hit
GTGTATATAGTAGCCGATGAAGGTGTAACCAGAAGCCTTGCAGGAGACCC20940.11769916677533511No Hit
GTTATAGACTCCCTTACACTTTTGGCCTGGGGACCAAGCTGGAGCTCAGT20870.11730571206309665No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20060.11275287896433726No Hit
GTAGTAATACTTGGCACTGTAGTCCAAGTTTGAGCCCACATCTCTCGCAC19920.11196596953986034No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19790.11123526793141746No Hit
GTCCAGGGACACGTCCATCAGTACAGCCTACATGGAGGTGAGCAGACTGT19740.11095422885124713No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18140.10196097828579652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.10173614702166024No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG17980.10106165322925145No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)