FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882052
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA29170.3307061261694322No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26370.29896196596119046No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21460.2432963135960238No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCGGTTTCACAAAGGCCTAT19590.22209574945694813No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18280.20724401735952078No Hit
GTCCTACAGTCCTCAGGACTCTATTCCCTCAGCAGCGTGGTGACCGTGCC17460.19794751329853566No Hit
GTATGATGGTTATCCCCTCACTTTCGGCGGAGGGACCAAGGTGGACATCA16640.18865100923755063No Hit
GTGTATTTACAAATGAAAAGCCTGAAAACCGAGGACTCAGCCGTGTATTT15860.17980799317954044No Hit
GTTGTACAGAAATACACGGCTGAGTCCTCGGTTTTCAGGCTTTTCATTTG15710.17810741316838463No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15480.17549985715127905No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT15310.17357253313863583No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15120.17141846512450512No Hit
CTGTACAACAGACCGGAGTTTTGGTTACTTCTTTAACTACTGGGGCCAGG15090.171078349122274No Hit
GGCCTATATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG14150.16042138105236425No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14130.1601946370508768No Hit
GTAAATACACCGTCTCTTTTGAATCATCCCTTGAGATGGTGAATCTGCCT13600.15418592101145964No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12690.1438690689437811No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12200.13831384090733878No Hit
CCATCATACTGTTGGCAGTAATAAGTTGCATAGTCATCAGGCTGCAGGCC12030.13638651689469555No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT11860.13445919288205233No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC11760.1333254728746151No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA11750.1332121008738714No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA11280.12788361683891653No Hit
ATTCAAAAGAGACGGTGTATTTACAAATGAAAAGCCTGAAAACCGAGGAC10800.12244176080321795No Hit
TCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACGAAGAC10500.11904060078090635No Hit
GTATTAGAGGTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT10470.11870048477867517No Hit
CTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10400.11790688077346913No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10280.1165464167645445No Hit
CCTCCACGCCATCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCCTGG10230.11597955676082589No Hit
ACTTATTACTGCCAACAGTATGATGGTTATCCCCTCACTTTCGGCGGAGG10170.11529932475636358No Hit
CTCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG10160.11518595275561985No Hit
GTTCAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10030.11371211674595148No Hit
GTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAG9980.11314525674223287No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.11167142073256452No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCG9740.1104243287243836No Hit
TCATGAGAGTGTCCTTGGGTTTTGGGGGGAACAGGAAGACTGATGGTCCC9670.10963072471917755No Hit
GCTCCAGGAGCACCTCTGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAG9660.10951735271843384No Hit
CTCCTGTGCAGCCTCTGGATTCGGTTTCACAAAGGCCTATATGAACTGGG9650.1094039807176901No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCGGGG9650.1094039807176901No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9610.10895049271471523No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAA9590.10872374871322779No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9550.1082702607102529No Hit
ATATAGGCCTTTGTGAAACCGAATCCAGAGGCTGCACAGGAGAGTCTAAG9530.10804351670876547No Hit
CTATAAGGCATCTACTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCA9510.10781677270727803No Hit
GTCTCCAACAAAGGCCTCCCGTCCTCCATCGAGAAAACCATCTCCAAAGC9430.10690979670132826No Hit
TATTTGGACTCAACTCTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATC9300.10543596069165989No Hit
ATATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTGGC9270.10509584468942874No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9240.10475572868719758No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTATTCCCTCAGCAGCGTG9200.1043022406842227No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA9160.10384875268124782No Hit
CTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTGAG9130.10350863667901665No Hit
CATTTACCCAGAGACAGGGAGAGGCTCTTCTGTGTGTAGTGGTTGTGCAG9060.1027150326738106No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8990.10192142866860457No Hit
ACTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAATGGATCTGGGAC8970.10169468466711712No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGAACTGCT8920.10112782466339854No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGA8870.10056096465967992No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGT8840.10022084865744876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14700.075.142871
TCAACGC18450.058.9198464
ATCAACG18750.057.977133
TATCAAC19800.054.974262
CAACGCA20050.054.2180145
AACGCAG20450.053.5095526
CAGCGCT5150.050.3241359
CGCAGAG22400.048.8513538
ACGCAGA22700.048.2057387
GCAGAGT26850.041.2911959
GCAGCGC7300.038.4611978
GGCAGCG8550.031.1542037
GTACATG22250.030.5008221
GTACACG9650.029.841025
ACATGGG21750.029.4585783
TACATGG22950.029.2110982
CATGGGA12250.028.2089044
TATGTAC10750.028.163622
CAGGGCA9750.027.3198414
GTATGTA11300.026.8339461
Produced by FastQC (version 0.11.5)