FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882052
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA27230.30871195802515045No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25110.28467709386748175No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18180.20611029735208355No Hit
GTCCTACAGTCCTCAGGACTCTATTCCCTCAGCAGCGTGGTGACCGTGCC15160.17187195312748No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14790.16767718909996235No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCGGTTTCACAAAGGCCTAT14720.16688358509475631No Hit
GTGTATTTACAAATGAAAAGCCTGAAAACCGAGGACTCAGCCGTGTATTT14120.1600812650501331No Hit
GTATGATGGTTATCCCCTCACTTTCGGCGGAGGGACCAAGGTGGACATCA13390.15180510899584151No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12450.1411481409259318No Hit
CTGTACAACAGACCGGAGTTTTGGTTACTTCTTTAACTACTGGGGCCAGG12140.1376336089028765No Hit
GTTGTACAGAAATACACGGCTGAGTCCTCGGTTTTCAGGCTTTTCATTTG12040.1364998888954393No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12000.1360464008924644No Hit
GGCCTATATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGG11770.13343884487535881No Hit
GTGGTGGACGTGAGCCAGGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11620.13173826486420304No Hit
GTAAATACACCGTCTCTTTTGAATCATCCCTTGAGATGGTGAATCTGCCT11230.12731675683519791No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11170.1266365248307356No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGA10810.12255513280396169No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10680.1210812967942933No Hit
CCATCATACTGTTGGCAGTAATAAGTTGCATAGTCATCAGGCTGCAGGCC10500.11904060078090635No Hit
GTCCTGCACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTC10440.11836036877644401No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGAACTGCT10020.11359874474520777No Hit
GTATTAGAGGTTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT9850.11167142073256452No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACATT9850.11167142073256452No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9590.10872374871322779No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA9560.10838363271099662No Hit
GTACGTGCTGTTGAACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9500.10770340070653431No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9390.10645630869835337No Hit
GTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAG9380.10634293669760968No Hit
ATATAGGCCTTTGTGAAACCGAATCCAGAGGCTGCACAGGAGAGTCTAAG9300.10543596069165989No Hit
GCTCCAGGAGCACCTCTGAGAGCACAGCCGCCCTGGGCTGCCTGGTCAAG8910.10101445266265481No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)